Journal article
Regulation of Cytokine-Induced iNOS Expression by a Hairpin Oligonucleotide in Murine Cerebral Endothelial Cells
Biochemical and biophysical research communications, Vol.235(2), pp.394-397
06/18/1997
DOI: 10.1006/bbrc.1997.6800
PMID: 9199204
Abstract
Inducible nitric oxide synthase (iNOS) is expressed in response to cytokines by a number of cell types participating in CNS inflammation, including brain cerebral endothelial cells. NF-κB, a transcription factor, mediates effector actions of pro-inflammatory cytokines. A combination of tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) enhanced the expression of iNOS in murine cerebral endothelial cells (MCECs). In an attempt to modulate TNF-α+IFN-γ induced expression of iNOS in MCECs, we designed a double-strand hairpin (hp) oligonucleotide carrying the NF-κB motif. This hp oligonucleotide inhibited NF-κB binding activity and decreased both iNOS mRNA and protein expression induced by TNF-α+IFN-γ. As a control, a mutant hp oligonucleotide was without effect. The present study confirms the role of transcription factor NF-κB in iNOS expression induced by TNF-α+IFN-γ in MCECs. More importantly, it demonstrates that an appropriately designed hp oligonucleotide is an effective tool to modulate iNOS expression and may be of potential pharmacological use.
Details
- Title: Subtitle
- Regulation of Cytokine-Induced iNOS Expression by a Hairpin Oligonucleotide in Murine Cerebral Endothelial Cells
- Creators
- Jan Xu - Department of Neurology, Washington University, School of Medicine, St. Louis, Missouri, 63110Yingji Wu - Department of Neurology, Washington University, School of Medicine, St. Louis, Missouri, 63110Luming He - Department of Neurology, Washington University, School of Medicine, St. Louis, Missouri, 63110Yanquing Yang - Department of Neurology, Washington University, School of Medicine, St. Louis, Missouri, 63110Steven A Moore - Department of Pathology, The University of Iowa, Iowa City, Iowa, 52242Chung Y Hsu - Department of Neurology, Washington University, School of Medicine, St. Louis, Missouri, 63110
- Resource Type
- Journal article
- Publication Details
- Biochemical and biophysical research communications, Vol.235(2), pp.394-397
- Publisher
- Elsevier Inc
- DOI
- 10.1006/bbrc.1997.6800
- PMID
- 9199204
- ISSN
- 0006-291X
- eISSN
- 1090-2104
- Language
- English
- Date published
- 06/18/1997
- Academic Unit
- Pathology
- Record Identifier
- 9984047994302771
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