Journal article
Regulation of GP63 mRNA stability in promastigotes of virulent and attenuated Leishmania chagasi
Molecular and biochemical parasitology, Vol.112(1), pp.51-59
2001
DOI: 10.1016/S0166-6851(00)00346-7
PMID: 11166386
Abstract
GP63 is a 63-kDa glycoprotein that is abundantly expressed on the surface of all
Leishmania species and is involved in several steps of promastigote infection of host cells.
Leishmania chagasi has at least 18 haploid
msp (major surface protease) genes encoding GP63 that are divided into three classes,
mspS,
mspL or
mspC, according to their unique 3′ UTR sequences and differential expression. All three
msp classes are constitutively transcribed during virulent promastigote growth in vitro, although
mspL mRNA is most abundant during logarithmic phase and
mspS mRNA predominates in stationary phase. Thus, the steady state levels of the
mspL and
mspS mRNAs are post-transcriptionally regulated. Using Actinomycin D to arrest transcription, we found that in virulent promastigotes the half-life (
t
1/2) of
mspL mRNA is coordinately modulated with growth phase, decreasing from a mean of 84 min during early logarithmic growth to a mean of 17 min at a stage intermediate between logarithmic and stationary phase. However, in attenuated promastigotes, the
t
1/2 of
mspL RNA remains the same throughout parasite growth. In contrast to
mspL RNA, the
t
1/2 of
mspS and
mspC RNA is constant throughout all growth phases of both virulent and attenuated promastigote growth. The presence of the translation inhibitor cycloheximide increases the
t
1/2 of
mspL RNA 4–6-fold in both virulent and attenuated promastigotes at all growth phases. These results indicate that the
t
1/2 of
mspL RNA is maintained by at least two distinct mechanisms — one activated during growth to stationary phase and the other dependent on a labile negative regulatory protein factor(s).
Details
- Title: Subtitle
- Regulation of GP63 mRNA stability in promastigotes of virulent and attenuated Leishmania chagasi
- Creators
- Andrew Brittingham - Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USAMelissa A Miller - Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USAJohn E Donelson - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USAMary E Wilson - Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Molecular and biochemical parasitology, Vol.112(1), pp.51-59
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0166-6851(00)00346-7
- PMID
- 11166386
- ISSN
- 0166-6851
- eISSN
- 1872-9428
- Language
- English
- Date published
- 2001
- Academic Unit
- Microbiology and Immunology; International Programs; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984002384502771
Metrics
19 Record Views