Journal article
Regulation of NOX1 expression by GATA, HNF-1α, and Cdx transcription factors
Free radical biology & medicine, Vol.44(3), pp.430-443
2008
DOI: 10.1016/j.freeradbiomed.2007.10.035
PMID: 18005670
Abstract
NOX1, a member of the NOX family of NADPH oxidases, is expressed primarily in colon epithelium, where it may function in host defense and growth regulation. We investigated factors responsible for its transcriptional regulation in vitro and its expression in vivo. Analysis of promoter constructs in the CaCo2 cell line identified a complex element between -
422 and -
291 critical for promoter activity. This element contained four sites that bound GATA-4, -5, and -6 in vitro with varied affinities. One site also bound the
caudal-related homeodomain proteins Cdx1 and Cdx2, whereas another also bound hepatocyte nuclear factor-1α (HNF-1α). GATA-6, HNF-1α, and Cdx2 also bound to this region in the intact chromatin of CaCo2 cells. These factors demonstrated cooperativity when transactivating the
NOX1 promoter.
NOX1 mRNA was detected in human colon epithelial cells along the crypt-villus axis. A gradient of
NOX1 mRNA expression was seen in the colons of normal as well as germ-free mice, with significantly higher levels in distal compared with proximal segments. The expression gradients of
NOX1 mRNA in the colon paralleled those of GATA-6, HNF-1α, and Cdx1. These data indicate that developmental, tissue-restricted transcription factors play a key role in
NOX1 regulation in vivo.
Details
- Title: Subtitle
- Regulation of NOX1 expression by GATA, HNF-1α, and Cdx transcription factors
- Creators
- Anthony J. Valente - The University of Texas Health Science Center at San AntonioQing Zhou - The University of Texas Health Science Center at San AntonioZhenhua Lu - The University of Texas Health Science Center at San AntonioWeijing He - The University of Texas Health Science Center at San AntonioMei Qiang - The University of Texas Health Science Center at San AntonioWuqiong Ma - The University of Texas Health Science Center at San AntonioGuiming Li - The University of Texas Health Science Center at San AntonioLong Wang - The University of Texas Health Science Center at San AntonioBotond Banfi - University of IowaKlaus Steger - Institute of Veterinary AnatomyKarl-Heinz Krause - University of GenevaRobert A. Clark - The University of Texas Health Science Center at San AntonioSenlin Li - The University of Texas Health Science Center at San Antonio
- Resource Type
- Journal article
- Publication Details
- Free radical biology & medicine, Vol.44(3), pp.430-443
- DOI
- 10.1016/j.freeradbiomed.2007.10.035
- PMID
- 18005670
- NLM abbreviation
- Free Radic Biol Med
- ISSN
- 0891-5849
- eISSN
- 1873-4596
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 2008
- Academic Unit
- Anatomy and Cell Biology; Otolaryngology; Internal Medicine
- Record Identifier
- 9984284359402771
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