Journal article
Regulation of Rev1 by the Fanconi anemia core complex
Nature structural & molecular biology, Vol.19(2), pp.164-170
02/01/2012
DOI: 10.1038/nsmb.2222
PMCID: PMC3280818
PMID: 22266823
Abstract
The 15 known Fanconi anemia proteins cooperate in a pathway that regulates DNA interstrand cross-link repair. Recent studies indicate that the Fanconi anemia pathway also controls Rev1-mediated translesion DNA synthesis (Us). We identified Fanconi anemia associated protein (FAAP20), an integral subunit of the multisubunit Fanconi anemia core complex. FAAP20 binds to FANCA subunit and is required for stability of the complex and monoubiquitination of FANCD2. FAAP20 contains a ubiquitin-binding zinc finger 4 domain and binds to the monoubiquitinated form of Rev1. FAAP20 binding stabilizes Rev1 nuclear foci and promotes interaction of the Fanconi anemia core with PCNA Rev1 DNA damage bypass complexes. FAAP20 therefore provides a critical link between the Fanconi anemia pathway and Us polymerase activity. We propose that the Fanconi anemia core complex regulates cross-link repair by channeling lesions to damage bypass pathways and preventing large DNA insertions and deletions.
Details
- Title: Subtitle
- Regulation of Rev1 by the Fanconi anemia core complex
- Creators
- Hyungjin Kim - Dana-Farber Cancer InstituteKailin Yang - Dana-Farber Cancer InstituteDonniphat Dejsuphong - Dana-Farber Cancer InstituteAlan D. D'Andrea - Dana Farber Canc Inst, Dept Radiat Oncol, Boston, MA 02115 USA
- Resource Type
- Journal article
- Publication Details
- Nature structural & molecular biology, Vol.19(2), pp.164-170
- Publisher
- Springer Nature
- DOI
- 10.1038/nsmb.2222
- PMID
- 22266823
- PMCID
- PMC3280818
- ISSN
- 1545-9993
- eISSN
- 1545-9985
- Number of pages
- 7
- Grant note
- R01DK043889 / NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) Leukemia and Lymphoma Society R37HL052725 / NATIONAL HEART, LUNG, AND BLOOD INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI)
- Language
- English
- Date published
- 02/01/2012
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984696721302771
Metrics
2 Record Views