Journal article
Regulation of Transcription Factor Pdr1p Function by an Hsp70 Protein in Saccharomyces cerevisiae
Molecular and cellular biology, Vol.18(3), pp.1147-1155
03/01/1998
DOI: 10.1128/MCB.18.3.1147
PMCID: PMC108827
PMID: 9488429
Abstract
Multiple or pleiotropic drug resistance in the yeast
Saccharomyces cerevisiae
requires the expression of several ATP binding cassette transporter-encoding genes under the control of the zinc finger-containing transcription factor Pdr1p. The ATP binding cassette transporter-encoding genes regulated by Pdr1p include
PDR5
and
YOR1
, which are required for normal cycloheximide and oligomycin tolerances, respectively. We have isolated a new member of the
PDR
gene family that encodes a member of the Hsp70 family of proteins found in this organism. This gene has been designated
PDR13
and is required for normal growth. Overexpression of Pdr13p leads to an increase in both the expression of
PDR5
and
YOR1
and a corresponding enhancement in drug resistance. Pdr13p requires the presence of both the
PDR1
structural gene and the Pdr1p binding sites in target promoters to mediate its effect on drug resistance and gene expression. A dominant, gain-of-function mutant allele of
PDR13
was isolated and shown to have the same phenotypic effects as when the gene is present on a 2μm plasmid. Genetic and Western blotting experiments indicated that Pdr13p exerts its effect on Pdr1p at a posttranslational step. These data support the view that Pdr13p influences pleiotropic drug resistance by enhancing the function of the transcriptional regulatory protein Pdr1p.
Details
- Title: Subtitle
- Regulation of Transcription Factor Pdr1p Function by an Hsp70 Protein in Saccharomyces cerevisiae
- Creators
- Timothy C. Hallstrom - University of IowaDavid J. Katzmann - #N#Program in Molecular Biology#N#1#N# andRodrigo J. Torres - University of IowaW. John Sharp - University of IowaW. Scott Moye-Rowley - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Molecular and cellular biology, Vol.18(3), pp.1147-1155
- Publisher
- American Society for Microbiology
- DOI
- 10.1128/MCB.18.3.1147
- PMID
- 9488429
- PMCID
- PMC108827
- ISSN
- 0270-7306
- eISSN
- 1098-5549
- Language
- English
- Date published
- 03/01/1998
- Academic Unit
- Radiology; Molecular Physiology and Biophysics; Surgery; Internal Medicine
- Record Identifier
- 9984297506502771
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