Regulation of Varicella-Zoster Virus-Induced Cell-to-Cell Fusion by the Endocytosis-Competent Glycoproteins gH and gE

Tracy Jo Pasieka; Lucie Maresova; Kimiyasu Shiraki; Charles Grose

doi:10.1128/JVI.78.6.2884-2896.2004

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Regulation of Varicella-Zoster Virus-Induced Cell-to-Cell Fusion by the Endocytosis-Competent Glycoproteins gH and gE

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Regulation of Varicella-Zoster Virus-Induced Cell-to-Cell Fusion by the Endocytosis-Competent Glycoproteins gH and gE

Tracy Jo Pasieka, Lucie Maresova, Kimiyasu Shiraki and Charles Grose

Journal of virology, Vol.78(6), pp.2884-2896

03/2004

DOI: 10.1128/JVI.78.6.2884-2896.2004

PMCID: PMC353742

PMID: 14990707

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Abstract

The gH glycoprotein of varicella-zoster virus (VZV) is a major fusogen. The realigned short cytoplasmic tail of gH (18 amino acids) harbors a functional endocytosis motif (YNKI) that mediates internalization in both VZV-infected and transfected cells (T. J. Pasieka, L. Maresova, and C. Grose, J. Virol. 77: 4194-4202, 2003). During subsequent confocal microscopy studies of endocytosis-deficient gH mutants, we observed that cells transfected with the gH tail mutants exhibited marked fusion. Therefore, we postulated that VZV gH endocytosis served to regulate cell-to-cell fusion. Subsequent analyses of gH+gL transfection fusion assays by the Kolmogorov-Smirnov statistical test demonstrated that expression of the endocytosis-deficient gH mutants resulted in a statistically significant enhancement of cell-to-cell fusion ( P < 0.0001) compared to wild-type gH. On the other hand, coexpression of VZV gE, another endocytosis-competent VZV glycoprotein, was able to temper the fusogenicity of the gH endocytosis mutants by facilitating internalization of the mutant gH protein from the cell surface. When the latter results were similarly analyzed, there was no longer any enhanced fusion by the endocytosis-deficient gH mutant protein. In summary, these studies support a role for gH endocytosis in regulating the cell surface expression of gH and thereby regulating gH-mediated fusion. The data also confirm and extend prior observations of a gE-gH interaction during viral glycoprotein trafficking in a VZV transfection system.

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Title: Subtitle: Regulation of Varicella-Zoster Virus-Induced Cell-to-Cell Fusion by the Endocytosis-Competent Glycoproteins gH and gE
Creators: Tracy Jo Pasieka - Department of Pediatrics, University of Iowa College of Medicine, Iowa City, Iowa
Lucie Maresova - Department of Pediatrics, University of Iowa College of Medicine, Iowa City, Iowa
Kimiyasu Shiraki - Department of Pediatrics, University of Iowa College of Medicine, Iowa City, Iowa
Charles Grose - Department of Pediatrics, University of Iowa College of Medicine, Iowa City, Iowa
Resource Type: Journal article
Publication Details: Journal of virology, Vol.78(6), pp.2884-2896
DOI: 10.1128/JVI.78.6.2884-2896.2004
PMID: 14990707
PMCID: PMC353742
NLM abbreviation: J Virol
ISSN: 0022-538X
eISSN: 1098-5514
Publisher: American Society for Microbiology
Language: English
Date published: 03/2004
Academic Unit: Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
Record Identifier: 9984093315502771

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