Journal article
Regulation of adenovirus transcription by an E1a gene in microinjected Xenopus laevis oocytes
Molecular and cellular biology, Vol.3(12), pp.2131-2142
1983
DOI: 10.1128/mcb.3.12.2131-2142.1983
PMCID: PMC370083
PMID: 6318084
Abstract
The regulation of adenovirus type 5 gene expression by the E1a gene product was examined in microinjected Xenopus laevis oocytes. Chimeric genes were constructed which included the promoter region of early adenovirus type 5 gene 3 and the structural sequence which codes for the bacterial enzyme chloramphenicol-3-O-acetyltransferase (CAT). A plasmid containing this chimeric gene as well as plasmids containing the E1a gene were coinjected into oocyte nuclei. The presence of the E1a gene was shown to increase CAT activity by up to 8.5-fold over basal levels. Synthesis of the functional product from the E1a gene requires the removal of intron sequences by RNA splicing. The E1a gene and a derivative that precisely lacks the intron were equally effective in increasing CAT activity, suggesting that splicing of the primary E1a transcript is efficiently accomplished in the oocyte nucleus. This was confirmed by directly examining the E1a mRNAs by the S1 mapping procedure. A protein extract from adenovirus type 5-infected HeLa cells enriched for the E1a protein may supplant the E1a plasmid in enhancing CAT activity. Synthesis of the CAT enzyme after gene injection is invariant in oocytes from the same frog, but oocytes from different frogs show a high degree of variability in their ability to synthesize the CAT enzyme. Microinjected X. laevis oocytes appear to be an extremely useful system to study the effects of protein elements on transcription.
Details
- Title: Subtitle
- Regulation of adenovirus transcription by an E1a gene in microinjected Xenopus laevis oocytes
- Creators
- N. C JONES - Purdue univ., dep. biological sciJ. D RICHTER - Purdue univ., dep. biological sciD. L WEEKS - Purdue univ., dep. biological sciL. D SMITH - Purdue univ., dep. biological sci
- Resource Type
- Journal article
- Publication Details
- Molecular and cellular biology, Vol.3(12), pp.2131-2142
- Publisher
- American Society for Microbiology; Washington, DC
- DOI
- 10.1128/mcb.3.12.2131-2142.1983
- PMID
- 6318084
- PMCID
- PMC370083
- ISSN
- 0270-7306
- eISSN
- 1098-5549
- Language
- English
- Date published
- 1983
- Academic Unit
- Stead Family Department of Pediatrics; Biochemistry and Molecular Biology
- Record Identifier
- 9984024405602771
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