Regulation of cell migration by the integrin beta subunit ectodomain

E J Filardo; S L Deming; D A Cheresh

doi:10.1242/jcs.109.6.1615

Back

Regulation of cell migration by the integrin beta subunit ectodomain

Journal article

Peer reviewed

Regulation of cell migration by the integrin beta subunit ectodomain

E J Filardo, S L Deming and D A Cheresh

Journal of cell science, Vol.109 (6), pp.1615-1622

06/1996

DOI: 10.1242/jcs.109.6.1615

PMID: 8799848

View Online

Abstract

CS-1 melanoma cells transfected with cDNAs encoding either the beta 3 or beta 5 integrin subunit protein express alpha v beta 3 or alpha v beta 5, respectively, enabling them to adhere to vitronectin yet only alpha v beta 3 promotes cell spreading and migration on this substrate. Following exposure to insulin or insulin-like growth factor, alpha v beta 5-expressing CS-1 cells gain the ability to migrate on vitronectin. To identify structural regions in beta 3 or beta 5 that account for these distinct biological properties, CS-1 cells were transfected with one of two chimeric beta subunit proteins, in which the ecto- and cytoplasmic domains of beta 3 and beta 5 were exchanged (termed alpha v beta 3/5 or alpha v beta 5/3). Surprisingly, alpha v beta 3/5 expressing cells spread and migrate on vitronectin while cells expressing alpha v beta 5/3 do not unless they are exposed to cytokine. These findings suggest that the distinct migratory properties mediated by integrins alpha v beta 3 and alpha v beta 5 and their response to cytokine activation is determined by a sequence(s) within the ectodomain of the integrin beta subunit.

Transfection

Humans

Integrins - chemistry

Platelet Membrane Glycoproteins - chemistry

Recombinant Fusion Proteins - physiology

Integrins - genetics

Antigens, CD - genetics

Cell Movement - physiology

Platelet Membrane Glycoproteins - physiology

Receptors, Vitronectin - physiology

Antigens, CD - chemistry

Molecular Structure

Receptors, Vitronectin - genetics

Tumor Cells, Cultured

Integrins - physiology

Cricetinae

Platelet Membrane Glycoproteins - genetics

Integrin beta3

Recombinant Fusion Proteins - chemistry

Vitronectin - metabolism

Animals

Cell Adhesion - physiology

Recombinant Fusion Proteins - genetics

Antigens, CD - physiology

Protein Conformation

Receptors, Vitronectin - chemistry

Integrin beta Chains

Details

Title: Subtitle: Regulation of cell migration by the integrin beta subunit ectodomain
Creators: E J Filardo - Department of Immunology, Scripps Research Institute, La Jolla, CA 92037, USA
S L Deming
D A Cheresh
Resource Type: Journal article
Publication Details: Journal of cell science, Vol.109 (6), pp.1615-1622
Publisher: England
DOI: 10.1242/jcs.109.6.1615
PMID: 8799848
ISSN: 0021-9533
eISSN: 1477-9137
Grant note: IF32CA59279 / NCI NIH HHS CA45726 / NCI NIH HHS CA50286 / NCI NIH HHS
Language: English
Date published: 06/1996
Academic Unit: Pharmaceutical Sciences and Experimental Therapeutics; Surgery; Internal Medicine
Record Identifier: 9984051704502771

Metrics

8 Record Views

32 Times Cited - Web of Science