Journal article
Regulation of chloride channels by protein kinase C in normal and cystic fibrosis airway epithelia
Science (American Association for the Advancement of Science), Vol.244(4910), pp.1353-1356
1989
DOI: 10.1126/science.2472006
PMID: 2472006
Abstract
Apical membrane chloride channels control chloride secretion by airway epithelial cells. Defective regulation of these channels is a prominent characteristic of cystic fibrosis. In normal intact cells, activation of protein kinase C (PKC) by phorbol ester either stimulated or inhibited chloride secretion, depending on the physiological status of the cell. In cell-free membrane patches, PKC also had a dual effect: at a high calcium concentration, PKC inactivated chloride channels; at a low calcium concentration, PKC activated chloride channels. In cystic fibrosis cells, PKC-dependent channel inactivation was normal, but activation was defective. Thus it appears that PKC phosphorylates and regulates two different sites on the channel or on an associated membrane protein, one of which is defective in cystic fibrosis.
Details
- Title: Subtitle
- Regulation of chloride channels by protein kinase C in normal and cystic fibrosis airway epithelia
- Creators
- Ming Li - Univ. Iowa coll. medicine, dep. internal medicine, Iowa City IA 52242, United StatesJohn D McCann - Univ. Iowa coll. medicine, dep. internal medicine, Iowa City IA 52242, United StatesMatthew P Anderson - Univ. Iowa coll. medicine, dep. internal medicine, Iowa City IA 52242, United StatesJohn P Clancy - Univ. Iowa coll. medicine, dep. internal medicine, Iowa City IA 52242, United StatesCarole M Liedtke - Univ. Iowa coll. medicine, dep. internal medicine, Iowa City IA 52242, United StatesAngus C Nairn - Univ. Iowa coll. medicine, dep. internal medicine, Iowa City IA 52242, United StatesPaul Greengard - Univ. Iowa coll. medicine, dep. internal medicine, Iowa City IA 52242, United StatesMichael J Welsh - Univ. Iowa coll. medicine, dep. internal medicine, Iowa City IA 52242, United States
- Resource Type
- Journal article
- Publication Details
- Science (American Association for the Advancement of Science), Vol.244(4910), pp.1353-1356
- Publisher
- American Association for the Advancement of Science; Washington, DC
- DOI
- 10.1126/science.2472006
- PMID
- 2472006
- ISSN
- 0036-8075
- eISSN
- 1095-9203
- Language
- English
- Date published
- 1989
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Biochemistry and Molecular Biology; Neurosurgery; Internal Medicine
- Record Identifier
- 9984020707202771
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