Journal article
Regulation of endothelial cell adhesion by profilin
Current biology, Vol.7(1), pp.24-30
01/1997
DOI: 10.1016/S0960-9822(06)00024-8
PMID: 9024619
Abstract
Background: Although profilin is believed to be an essential regulator of the actin cytoskeleton in most cells, its precise role in mammalian cells remains unknown. We have used replication-incompetent adenovirus carrying the human profilin I cDNA as a means rapidly to increase the concentration of profilin in human aortic endothelial cells 12–31-fold above baseline – levels never before achieved in mammalian cells.
Results: The concentration of filamentous actin was not detectably affected by profilin overexpression. Actin stress fibers were, however, absent from areas of high profilin content in overexpressing cells, and the bulk of filaments was located at the periphery of the cells. We observed a gradient in the distribution of overexpressed profilin in migrating endothelial cells, with most profilin molecules concentrated near the advancing edge where focal contacts are being formed and focal adhesion proteins are located. Profilin overexpression resulted in increased recruitment of fibronectin receptors to the plasma membrane. Adhesion of endothelial cells to fibronectin was markedly and selectively increased by profilin overexpression.
Conclusions: We conclude that an important role for profilin in mammalian cells may be its contribution to the formation of focal contacts, particularly those involving the fibronectin receptor.
Details
- Title: Subtitle
- Regulation of endothelial cell adhesion by profilin
- Creators
- Nicanor I Moldovan - Department of Medicine, Cardiology Division, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USAEmily E Milliken - Department of Medicine, Cardiology Division, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USAKaikobad Irani - Department of Medicine, Cardiology Division, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USAJie Chen - Department of Medicine, Cardiology Division, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USARichard H Sohn - Department of Medicine, Cardiology Division, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USAToren Finkel - Cardiology Branch, NHLBI, 10/7B15, NIH, Bethesda, Maryland 20892-1650, USAPascal J Goldschmidt-Clermont - Department of Medicine, Department of Cell Biology and Anatomy, Cardiology Division, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
- Resource Type
- Journal article
- Publication Details
- Current biology, Vol.7(1), pp.24-30
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S0960-9822(06)00024-8
- PMID
- 9024619
- ISSN
- 0960-9822
- eISSN
- 1879-0445
- Language
- English
- Date published
- 01/1997
- Academic Unit
- Cardiovascular Medicine; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
- Record Identifier
- 9984046809602771
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