Regulation of hepatic glycogenolysis and vasoconstriction during antigen-induced anaphylaxis

Keith L Hines; Rory A Fisher

doi:10.1152/ajpgi.1992.262.5.G868

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Regulation of hepatic glycogenolysis and vasoconstriction during antigen-induced anaphylaxis

Journal article

Peer reviewed

Regulation of hepatic glycogenolysis and vasoconstriction during antigen-induced anaphylaxis

Keith L Hines and Rory A Fisher

The American journal of physiology, Vol.262(5 Pt 1), pp.G868-G877

05/1992

DOI: 10.1152/ajpgi.1992.262.5.G868

PMID: 1375439

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Abstract

Effects of sensitizing antigen (ovalbumin) on various physiological and hepatic parameters were investigated in sensitized rats and isolated perfused livers derived from sensitized rats. Administration of ovalbumin (500 micrograms) to the portal venous circulation of sensitized but not nonsensitized rats resulted in a rapid and sustained decrease in systemic arterial pressure, characteristic of antigen-induced anaphylaxis, and pronounced increases in hepatic portal pressure and blood glucose concentration. These antigen-mediated alterations were similar to those observed in response to platelet-activating factor (PAF) (0.1 micrograms/kg) administration to rats and were inhibited significantly by specific PAF receptor antagonist WEB 2086 (250 micrograms/kg). Infusion of ovalbumin (3.8 micrograms/ml) into isolated perfused livers derived from sensitized rats resulted in significant increases in hepatic glucose output and portal pressure and decreases in oxygen consumption, as observed in response to PAF (0.28 nM) infusion into perfused livers. These hepatic responses to ovalbumin were antigen specific and were not observed in nonsensitized rat perfused livers. Hemodynamic and glycogenolytic responses to ovalbumin in perfused livers were inhibited significantly but less effectively than similar responses to PAF by infusion of WEB 2086 (500 nM) into livers. Coinfusion of indomethacin (2.8 microM) and nordihydroguariatic acid (1 microM) with WEB 2086 (500 nM) into perfused livers inhibited further hemodynamic but not glycogenolytic responses to ovalbumin. Infusion of nitric oxide (34 microM) into sensitized rat perfused livers prevented the hemodynamic and glycogenolytic responses to both ovalbumin and PAF. These observations provide evidence that hepatic glycogenolysis and vasoconstriction are stimulated during antigen-induced anaphylaxis and suggest that these responses are mediated in part by PAF.

Nitric Oxide - pharmacology

Liver - metabolism

Platelet Activating Factor - physiology

Platelet Activating Factor - antagonists & inhibitors

Rats

Eicosanoids - antagonists & inhibitors

Epitopes

Ovalbumin - pharmacology

Rats, Inbred Strains

Vasoconstriction - drug effects

Azepines - pharmacology

Triazoles - pharmacology

Animals

Glycogen - metabolism

Anaphylaxis - physiopathology

Anaphylaxis - immunology

Antigens - immunology

Female

Anaphylaxis - metabolism

Details

Title: Subtitle: Regulation of hepatic glycogenolysis and vasoconstriction during antigen-induced anaphylaxis
Creators: Keith L Hines - Department of Pharmacology, College of Medicine, University of Iowa, Iowa City 52242
Rory A Fisher
Resource Type: Journal article
Publication Details: The American journal of physiology, Vol.262(5 Pt 1), pp.G868-G877
DOI: 10.1152/ajpgi.1992.262.5.G868
PMID: 1375439
NLM abbreviation: Am J Physiol
ISSN: 0002-9513
eISSN: 2163-5773
Publisher: American Physiological Society; United States
Grant note: HL-41071 / NHLBI NIH HHS DK-25295 / NIDDK NIH HHS
Language: English
Date published: 05/1992
Academic Unit: Iowa Neuroscience Institute; Neuroscience and Pharmacology; Internal Medicine
Record Identifier: 9984040449502771

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