Regulation of hippocampal synapse remodeling by epileptiform activity

Xiang-Ming Zha; Steven H Green; Michael E Dailey

doi:10.1016/j.mcn.2005.04.007

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Regulation of hippocampal synapse remodeling by epileptiform activity

Journal article

Peer reviewed

Regulation of hippocampal synapse remodeling by epileptiform activity

Xiang-Ming Zha, Steven H Green and Michael E Dailey

Molecular and cellular neurosciences, Vol.29(4), pp.494-506

08/2005

DOI: 10.1016/j.mcn.2005.04.007

PMID: 15953736

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Abstract

We examined the regulation of dendritic spines and synapses by epileptiform activity (EA) in rat hippocampal slice cultures. EA, which was induced by a GABA(A) receptor inhibitor, gabazine, reduced pyramidal neuron spine density by approximately 50% after 48 h and also caused an increase in the average length of remaining spines. To directly determine the effects of EA on synapses, we used fluorescent protein-tagged PSD95, which marks postsynaptic densities. EA induced a net loss of synapses on spines but not shafts; conversely, activity blockade (TTX) induced a loss of shaft synapses. Time-lapse confocal imaging in live tissue slices revealed that EA (1) shifts the balance of synapse gain and loss in dendrites leading to a net loss of spine synapses and (2) induces the formation of new filopodia-like dendritic structures having abnormally slow motility. These results identify EA-induced changes in the density and distribution of synaptic structures on dendrites.

Disks Large Homolog 4 Protein

Synaptic Transmission - physiology

Neuronal Plasticity - drug effects

Intracellular Signaling Peptides and Proteins - metabolism

Epilepsy - physiopathology

Hippocampus - drug effects

Pyridazines - pharmacology

Synapses - pathology

Pseudopodia - pathology

Pseudopodia - drug effects

Neuronal Plasticity - physiology

Dendritic Spines - drug effects

Synaptic Transmission - drug effects

Membrane Proteins - metabolism

Organ Culture Techniques

Animals, Newborn

Synapses - drug effects

Synaptic Membranes - pathology

Synapses - physiology

Dendritic Spines - physiology

Rats

Sodium Channel Blockers - pharmacology

Hippocampus - pathology

Down-Regulation - drug effects

GABA Antagonists - pharmacology

Down-Regulation - physiology

Synaptic Membranes - drug effects

Animals

Guanylate Kinases

Epilepsy - chemically induced

Dendritic Spines - pathology

Pseudopodia - physiology

Epilepsy - pathology

Hippocampus - physiopathology

Synaptic Membranes - metabolism

Details

Title: Subtitle: Regulation of hippocampal synapse remodeling by epileptiform activity
Creators: Xiang-Ming Zha - Department of Biological Sciences, University of Iowa, Iowa City, IA 52242-1324, USA
Steven H Green
Michael E Dailey
Resource Type: Journal article
Publication Details: Molecular and cellular neurosciences, Vol.29(4), pp.494-506
Publisher: United States
DOI: 10.1016/j.mcn.2005.04.007
PMID: 15953736
ISSN: 1044-7431
eISSN: 1095-9327
Grant note: R29 NS037159 / NINDS NIH HHS NS37159 / NINDS NIH HHS DC02961 / NIDCD NIH HHS R01 DC002961 / NIDCD NIH HHS
Language: English
Date published: 08/2005
Academic Unit: Iowa Neuroscience Institute; Biology; Otolaryngology
Record Identifier: 9984070513602771

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