Regulation of parathyroid hormone-related protein expression in a canine squamous carcinoma cell line by colchicine

A Gröne; M T Weckmann; C C Capen; T J Rosol

doi:10.1016/S0940-2993(98)80017-1

Back

Regulation of parathyroid hormone-related protein expression in a canine squamous carcinoma cell line by colchicine

Journal article

Peer reviewed

Regulation of parathyroid hormone-related protein expression in a canine squamous carcinoma cell line by colchicine

A Gröne, M T Weckmann, C C Capen and T J Rosol

Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, Vol.50(4-6), pp.365-370

09/1998

DOI: 10.1016/S0940-2993(98)80017-1

PMID: 9784008

View Online

Abstract

The regulation of parathyroid hormone-related protein expression by colchicine, vinblastine, nocodazole, taxol, transforming growth factor-beta1 (TGFbeta1), and epidermal growth factor (EGF) was investigated in a canine squamous carcinoma cell line (SCC 2/88 cells). SCC 2/88 cells were stably transfected with a human P2/P3 PTHrP promoter-luciferase reporter gene construct and gene expression was measured after chemical treatments. The greatest increase in reporter gene expression was observed after colchicine treatment and small increases occurred after treatment with vinblastine, taxol, TGFbeta1, or EGF. Nocodazole had no significant effect on reporter gene expression. Colchicine also increased PTHrP steady state mRNA expression and PTHrP secretion by SCC 2/88 cells. These results demonstrated that PTHrP production was increased in SCC 2/88 cells by colchicine and suggested that factors or events during mitosis are capable of stimulating PTHrP production. An increase in PTHrP production during mitosis of malignant epithelial cells may be important in the pathogenesis of humoral hypercalcemia of malignancy.

Animals

Blotting, Northern

Carcinoma, Squamous Cell - drug therapy

Carcinoma, Squamous Cell - metabolism

Colchicine - pharmacology

Dogs

Epidermal Growth Factor - pharmacology

Gene Expression Regulation - drug effects

Luciferases - metabolism

Lumicolchicines - pharmacology

Neoplasm Proteins - genetics

Neoplasm Proteins - metabolism

Nocodazole - pharmacology

Paclitaxel - pharmacology

Parathyroid Hormone-Related Protein

Proteins - genetics

Proteins - metabolism

Radioimmunoassay

RNA, Messenger - metabolism

Transcription, Genetic

Transfection

Transforming Growth Factor beta - pharmacology

Tumor Cells, Cultured - drug effects

Tumor Cells, Cultured - metabolism

Vinblastine - pharmacology

Details

Title: Subtitle: Regulation of parathyroid hormone-related protein expression in a canine squamous carcinoma cell line by colchicine
Creators: A Gröne - The Ohio State University
M T Weckmann - The Ohio State University
C C Capen - The Ohio State University
T J Rosol - The Ohio State University
Resource Type: Journal article
Publication Details: Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, Vol.50(4-6), pp.365-370
DOI: 10.1016/S0940-2993(98)80017-1
PMID: 9784008
ISSN: 0940-2993
eISSN: 1618-1433
Grant note: AR40220 / NIAMS NIH HHS AR01923 / NIAMS NIH HHS
Language: English
Date published: 09/1998
Academic Unit: Psychiatry; Family and Community Medicine
Record Identifier: 9984297445802771

Metrics

12 Record Views

3 Times Cited - Web of Science