Journal article
Regulation of proteasome activity in activated human platelets
Cell calcium (Edinburgh), Vol.49(4), pp.226-232
04/01/2011
DOI: 10.1016/j.ceca.2011.02.005
PMID: 21388679
Abstract
Ubiquitin-proteasome system has emerged a central player in regulation of diverse cellular processes. However, relevance of proteasome activity in platelets, which are terminally differentiated enucleate cells, is not clear. In this report we show that activation of platelets with physiological agonists was associated with 7-10 -fold rise in proteasomal activity. Elevation of cytosolic calcium with A23187 or thapsigargin resulted in significant increase in enzymatic activity, while treatment with intracellular calcium chelator or inhibitor of inositol trisphosphate receptor attenuated proteasomal enzymes in collagen-stimulated platelets. Specific inhibitors of protein kinase C as well as calpain, too, downregulated proteasome function. To conclude, proteasomal enzymatic activity in platelets is regulated by cytosolic calcium through Ca(2+)-dependent downstream effectors like calpain and protein kinase C. (C) 2011 Elsevier Ltd. All rights reserved.
Details
- Title: Subtitle
- Regulation of proteasome activity in activated human platelets
- Creators
- Manasa K. Nayak - Banaras Hindu UniversityKailash Kumar - Banaras Hindu UniversityDebabrata Dash - Banaras Hindu University
- Resource Type
- Journal article
- Publication Details
- Cell calcium (Edinburgh), Vol.49(4), pp.226-232
- Publisher
- Elsevier
- DOI
- 10.1016/j.ceca.2011.02.005
- PMID
- 21388679
- ISSN
- 0143-4160
- eISSN
- 1532-1991
- Number of pages
- 7
- Grant note
- Council of Scientific and Industrial Research (CSIR); Council of Scientific & Industrial Research (CSIR) - India Department of Science and Technology (DST), Government of India; Department of Science & Technology (India) Department of Biotechnology (DBT); Department of Biotechnology (DBT) India
- Language
- English
- Date published
- 04/01/2011
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984359885102771
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