Journal article
Regulation of sodium absorption by canine tracheal epithelium
The Journal of clinical investigation, Vol.79(1), pp.73-79
1987
DOI: 10.1172/JCI112811
PMID: 3098785
Abstract
To regulate the quantity of respiratory tract fluid, the airway epithelium either secretes chloride, Cl-, or reabsorbs sodium, Na+. Many secretagogues inhibit Na+ absorption, but the decrease may result from a fall in the electrochemical gradient for Na+ absorption. We examined regulation of Na+ absorption independent of Cl- secretion, by bathing canine tracheal epithelium in Cl--free, gluconate Ringers solution. Prostaglandin E2, 2-chloroadenosine, and isoproterenol increased short-circuit current (Isc) and the rate of Na+ absorption. In contrast, indomethacin, which inhibits endogenous prostaglandin production, decreased Isc. These agents regulate cellular levels of cAMP; direct addition of 8-Br-cAMP also acutely increased Isc. We examined chronic regulation of Na+ absorption in cell monolayers grown on permeable supports in serum-free media. Exposure to aldosterone for two days increased baseline Isc by 50% and the amiloride-inhibitable current by 55%. These data indicate that Na+ absorption is both acutely and chronically regulated in the airway epithelium.
Details
- Title: Subtitle
- Regulation of sodium absorption by canine tracheal epithelium
- Creators
- J. J CULLEN - Univ. Iowa coll. medicine, dep. internal medicine, Iowa City IA 52242, United StatesM. J WELSH - Univ. Iowa coll. medicine, dep. internal medicine, Iowa City IA 52242, United States
- Resource Type
- Journal article
- Publication Details
- The Journal of clinical investigation, Vol.79(1), pp.73-79
- Publisher
- American Society for Clinical Investigation
- DOI
- 10.1172/JCI112811
- PMID
- 3098785
- ISSN
- 0021-9738
- eISSN
- 1558-8238
- Language
- English
- Date published
- 1987
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Surgery; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center; Neurosurgery; Internal Medicine
- Record Identifier
- 9984020765502771
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