Journal article
Regulation of surfactant protein D in the mouse female reproductive tract in vivo
Molecular human reproduction, Vol.13(12), pp.863-868
12/2007
DOI: 10.1093/molehr/gam074
PMID: 17954522
Abstract
Surfactant protein D (SP-D) plays a role in innate immunity in the lung and is expressed at many other mucosal surfaces throughout the human body. In this study, we show that SP-D mRNA and protein are present in the murine female reproductive tract; i.e. in the vagina, cervix, uterus and oviduct. SP-D protein is primarily localized to epithelial cells lining the genital tract and is also present in secretory material within the lumen of the uterus and cervix. The levels of SP-D mRNA in the uterus vary by a factor of 10 during the estrous cycle with peak levels present at estrus and the lowest levels at diestrus. In contrast, SP-D mRNA levels in the lung do not change during the estrous cycle. Since SP-D is an innate host defense protein present in the mouse reproductive tract, we studied the influence of infection on SP-D levels in vivo. We found that Chlamydia muridarum infection caused an increase in the SP-D protein content of reproductive tract epithelial cells. These data are suggestive that SP-D may play a role in innate immunity in the female reproductive tract in vivo.
Details
- Title: Subtitle
- Regulation of surfactant protein D in the mouse female reproductive tract in vivo
- Creators
- Rebecca E Oberley - 1 Department of Medicine, National Jewish Medical and Research Center, Denver, CO 80206, USAKelli L Goss - 2 Department of Anatomy and Cell Biology, University of Iowa College of Medicine, Iowa City, IA 52242, USADarren S Hoffmann - 2 Department of Anatomy and Cell Biology, University of Iowa College of Medicine, Iowa City, IA 52242, USAKevin A Ault - 4 Department of Obstetrics and Gynecology, University of Iowa College of Medicine, Iowa City, IA 52242, USATraci L Neff - 4 Department of Obstetrics and Gynecology, University of Iowa College of Medicine, Iowa City, IA 52242, USAKyle H Ramsey - 5 Department of Microbiology and Immunology, Chicago College of Osteopathic Medicine, Midwestern University, Downers Grove, IL 60515, USAJeanne M Snyder - 2 Department of Anatomy and Cell Biology, University of Iowa College of Medicine, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Molecular human reproduction, Vol.13(12), pp.863-868
- Publisher
- Oxford University Press
- DOI
- 10.1093/molehr/gam074
- PMID
- 17954522
- ISSN
- 1360-9947
- eISSN
- 1460-2407
- Language
- English
- Date published
- 12/2007
- Academic Unit
- Oral Pathology, Radiology and Medicine; Anatomy and Cell Biology; Stead Family Department of Pediatrics; Internal Medicine
- Record Identifier
- 9984025683202771
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