Journal article
Regulation of surfactant protein and defensin mRNA expression in cultured ovine type II pneumocytes by all-trans retinoic acid and VEGF
International journal of experimental pathology, Vol.87(5), pp.393-403
Received for publication: 30 January 2006 Accepted for publication: 14 June 2006
10/2006
DOI: 10.1111/j.1365-2613.2006.00494.x
PMCID: PMC2517377
PMID: 16965567
Abstract
Beta-defensins and surfactant proteins are components of the pulmonary innate immune system. Their gene expression is regulated by development, hormones, growth and immunoregulatory factors. It was our hypothesis that growth and differentiation factors such as all-trans retinoic acid (RA) and vascular endothelial growth factor (VEGF) may affect expression of selected innate immune genes by respiratory epithelial cells. Ovine JS7 cells (alveolar type II pneumocytes) were incubated in serum-free Dulbecco's modified Eagle's medium (DMEM) complete media that contained: no treatment (negative control), RA (500 nM), or VEGF (100 ng/ml) for 6, 12 or 24 h incubation. Total RNA was isolated, cDNA synthesized, and relative mRNA levels of surfactant protein A (SP-A) and SP-D, and sheep beta-defensin-1 (SBD-1) were determined by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Cells had significantly increased expression of SP-D mRNA at 6 h and 24 h, decreased expression of SP-A mRNA at 12 h, and unchanged levels of SBD-1 mRNA after the treatment with RA compared with their respective negative controls. VEGF did not alter the expression of the three innate immune genes. These findings suggest that SP-A and SP-D have different transcription regulation pathways, and that expression of SBD-1 is not inducible by RA similar to its human homolog HBD-1. The lack of changes induced by VEGF treatment suggests that VEGF does not have a direct effect on epithelial cells, but may affect gene expression indirectly.
Details
- Title: Subtitle
- Regulation of surfactant protein and defensin mRNA expression in cultured ovine type II pneumocytes by all-trans retinoic acid and VEGF
- Creators
- B Grubor - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USAD. K Meyerholz - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USAT Lazic - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USAM. M DeMacedo - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USAR. J Derscheid - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USAJ. M Hostetter - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USAJ. M Gallup - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USAJ. C DeMartini - Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, USAM. R Ackermann - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USA
- Resource Type
- Journal article
- Publication Details
- International journal of experimental pathology, Vol.87(5), pp.393-403
- Edition
- Received for publication: 30 January 2006 Accepted for publication: 14 June 2006
- Publisher
- Blackwell Publishing Ltd
- DOI
- 10.1111/j.1365-2613.2006.00494.x
- PMID
- 16965567
- PMCID
- PMC2517377
- ISSN
- 0959-9673
- eISSN
- 1365-2613
- Number of pages
- 11
- Language
- English
- Date published
- 10/2006
- Academic Unit
- Pathology
- Record Identifier
- 9984083892602771
Metrics
19 Record Views