Journal article
Regulation of the Nanog Gene by Both Positive and Negative cis-Regulatory Elements in Embryonal Carcinoma Cells and Embryonic Stem Cells
Molecular reproduction and development, Vol.76(2), pp.173-182
02/2009
DOI: 10.1002/mrd.20943
PMCID: PMC2614456
PMID: 18537119
Abstract
The transcription factor Nanog is essential for mammalian embryogenesis, as well as the pluripotency of embryonic stem (ES) cells. Work with ES cells and embryonal carcinoma (EC) cells previously identified positive and negative
cis
-regulatory elements that influence the activity of the Nanog promoter, including adjacent
cis
-regulatory elements that bind Sox2 and Oct-3/4. Given the importance of Nanog during mammalian development, we examined the
cis
-regulatory elements required for Nanog promoter activity more closely. In this study, we demonstrate that two positive
cis
-regulatory elements previously shown to be active in F9 EC cells are also active in ES cells. We also identify a novel negative regulatory region that is located in close proximity to two other positive Nanog
cis
-regulatory elements. Although this negative regulatory region is active in F9 EC cells and ES cells, it is inactive in P19 EC cells. Furthermore, we demonstrate that one of the positive
cis
-regulatory elements active in F9 EC cells and ES cells is inactive in P19 EC cells. Together, these and other studies suggest that Nanog transcription is regulated by the interplay of positive and negative
cis
-regulatory elements. Given that P19 appears to be more closely related to a later developmental stage of mammalian development than F9 and ES cells, differential utilization of
cis
-regulatory elements may reflect mechanisms used during development to achieve the correct level of Nanog expression as embryogenesis unfolds.
Details
- Title: Subtitle
- Regulation of the Nanog Gene by Both Positive and Negative cis-Regulatory Elements in Embryonal Carcinoma Cells and Embryonic Stem Cells
- Creators
- Brian Boer - Eppley Institute for Research in Cancer and Allied Diseases University of Nebraska Medical Center 986805 Nebraska Medical Center Omaha, Nebraska 68198-6805Jesse L Cox - Eppley Institute for Research in Cancer and Allied Diseases University of Nebraska Medical Center 986805 Nebraska Medical Center Omaha, Nebraska 68198-6805David Claassen - Eppley Institute for Research in Cancer and Allied Diseases University of Nebraska Medical Center 986805 Nebraska Medical Center Omaha, Nebraska 68198-6805Sunil Kumar Mallanna - Eppley Institute for Research in Cancer and Allied Diseases University of Nebraska Medical Center 986805 Nebraska Medical Center Omaha, Nebraska 68198-6805Michelle Desler - Eppley Institute for Research in Cancer and Allied Diseases University of Nebraska Medical Center 986805 Nebraska Medical Center Omaha, Nebraska 68198-6805Angie Rizzino - Eppley Institute for Research in Cancer and Allied Diseases University of Nebraska Medical Center 986805 Nebraska Medical Center Omaha, Nebraska 68198-6805
- Resource Type
- Journal article
- Publication Details
- Molecular reproduction and development, Vol.76(2), pp.173-182
- DOI
- 10.1002/mrd.20943
- PMID
- 18537119
- PMCID
- PMC2614456
- NLM abbreviation
- Mol Reprod Dev
- ISSN
- 1040-452X
- eISSN
- 1098-2795
- Language
- English
- Date published
- 02/2009
- Academic Unit
- Stead Family Department of Pediatrics; Hematology/Oncology
- Record Identifier
- 9984093362502771
Metrics
9 Record Views