Journal article
Regulation of the Phosphorylation State and Microtubule-Binding Activity of Tau by Protein Phosphatase 2A
Neuron (Cambridge, Mass.), Vol.17(6), pp.1201-1207
12/1996
DOI: 10.1016/S0896-6273(00)80250-0
PMID: 8982166
Abstract
Recently, we reported that a pool of protein phosphatase 2A (PP2A) is associated with microtubules. Here, we demonstrate that specific isoforms of PP2A bind and dephosphorylate the neuronal microtubule-associated protein tau. Coexpression of tau and SV40 small t, a specific inhibitor of PP2A, in CV-1, NIH 3T3, or NT2 cells induced the phosphorylation of tau at multiple sites, including Ser-199, Ser-202, Thr-205, Ser-396, and Ser-404. Immunofluorescent and biochemical analyses revealed that hyperphosphorylation correlated with dissociation of tau from microtubules and a loss of tau-induced microtubule stabilization. Taken together, these results support the hypothesis that PP2A controls the phosphorylation state of tau in vivo.
Details
- Title: Subtitle
- Regulation of the Phosphorylation State and Microtubule-Binding Activity of Tau by Protein Phosphatase 2A
- Creators
- Estelle Sontag - Department of Pharmacology, University of Texas, Southwestern Medical Center, Dallas, Texas 75235, USAViyada Nunbhakdi-Craig - Department of Pharmacology, University of Texas, Southwestern Medical Center, Dallas, Texas 75235, USAGloria Lee - Center for Neurologic Diseases, Brigham and Women's Hospital, and Harvard Medical School, Boston, Massachusetts 02115, USAGeorge S Bloom - Department of Cell Biology and Neuroscience, University of Texas, Southwestern Medical Center, Dallas, Texas 75235, USAMarc C Mumby - Department of Pharmacology, University of Texas, Southwestern Medical Center, Dallas, Texas 75235, USA
- Resource Type
- Journal article
- Publication Details
- Neuron (Cambridge, Mass.), Vol.17(6), pp.1201-1207
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S0896-6273(00)80250-0
- PMID
- 8982166
- ISSN
- 0896-6273
- eISSN
- 1097-4199
- Language
- English
- Date published
- 12/1996
- Academic Unit
- Iowa Neuroscience Institute; Immunology; Internal Medicine
- Record Identifier
- 9984065377802771
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