Regulatory effects of dioxin-like and non-dioxin-like PCBs and other AhR ligands on the antioxidant enzymes paraoxonase 1/2/3

Hua Shen; Larry Robertson; Gabriele Ludewig

doi:10.1007/s11356-015-4722-1

Back

Regulatory effects of dioxin-like and non-dioxin-like PCBs and other AhR ligands on the antioxidant enzymes paraoxonase 1/2/3

Journal article

Peer reviewed

Regulatory effects of dioxin-like and non-dioxin-like PCBs and other AhR ligands on the antioxidant enzymes paraoxonase 1/2/3

Hua Shen, Larry Robertson and Gabriele Ludewig

Environmental science and pollution research international, Vol.23(3), pp.2108-2118

02/2016

DOI: 10.1007/s11356-015-4722-1

PMCID: PMC4662644

PMID: 26006071

View Online

Abstract

Paraoxonase 1 (PON1), an antioxidant enzyme, is believed to play a critical role in many diseases, including cancer. PCBs are widespread environmental contaminants known to induce oxidative stress and cancer and to produce changes in gene expression of various pro-oxidant and antioxidant enzymes. Thus, it appeared of interest to explore whether PCBs may modulate the activity and/or gene expression of PON1 as well. In this study, we compared the effects of dioxin-like and non-dioxin-like PCBs and of various aryl hydrocarbon receptor (AhR) ligands on PON1 regulation and activity in male and female Sprague-Dawley rats. Our results demonstrate that (i) the non-dioxin-like PCB154, PCB155, and PCB184 significantly reduced liver and serum PON1 activities, but only in male rats; (ii) the non-dioxin-like PCB153, the most abundant PCB in many matrices, did not affect PON1 messenger RNA (mRNA) level in the liver but significantly decreased serum PON1 activity in male rats; (iii) PCB126, an AhR ligand and dioxin-like PCB, increased both PON1 activities and gene expression; and (iv) even though three tested AhR ligands induced CYP1A in several tissues to a similar extent, they displayed differential effects on the three PONs and AhR, i.e., PCB126 was an efficacious inducer of PON1, PON2, PON3, and AhR in the liver, while 3-methylcholantrene induced liver AhR and lung PON3, and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), the most potent AhR agonist, increased only PON3 in the lung, at the doses and exposure times used in these studies. These results show that PCBs may have an effect on the antioxidant protection by paraoxonases in exposed populations and that regulation of gene expression through AhR is highly diverse.

Environmental Chemistry

Environmental Health

PON3

Ecotoxicology

PON2

PON1

3-MC

TCDD

AhR

Environment, general

PCB126

Non-dioxin-like PCB

Environment

Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution

Atmospheric Protection/Air Quality Control/Air Pollution

ISRP Project 1 2015-2020

Synthesis Core

Details

Title: Subtitle: Regulatory effects of dioxin-like and non-dioxin-like PCBs and other AhR ligands on the antioxidant enzymes paraoxonase 1/2/3
Creators: Hua Shen - Interdisciplinary Graduate Program in Human Toxicology, Graduate College The University of Iowa Iowa City IA USA
Larry Robertson - Department of Occupational & Environmental Health, College of Public Health The University of Iowa 100 Oakdale Campus, 214 IREH Iowa City IA 52242-5000 USA
Gabriele Ludewig - Department of Occupational & Environmental Health, College of Public Health The University of Iowa 100 Oakdale Campus, 214 IREH Iowa City IA 52242-5000 USA
Resource Type: Journal article
Publication Details: Environmental science and pollution research international, Vol.23(3), pp.2108-2118
DOI: 10.1007/s11356-015-4722-1
PMID: 26006071
PMCID: PMC4662644
NLM abbreviation: Environ Sci Pollut Res Int
ISSN: 0944-1344
eISSN: 1614-7499
Publisher: Springer Berlin Heidelberg; Berlin/Heidelberg
Language: English
Date published: 02/2016
Academic Unit: Occupational and Environmental Health; Iowa Superfund Research Program
Record Identifier: 9983997365602771

Metrics

24 Record Views

12 Times Cited - Web of Science