Journal article
Regulatory sequences and a novel gene in the msp (GP63) gene cluster of Leishmania chagasi
Molecular and biochemical parasitology, Vol.95(2), pp.251-265
1998
DOI: 10.1016/S0166-6851(98)00112-1
PMID: 9803417
Abstract
The surface protease GP63 of
Leishmania chagasi is encoded by a cluster of more than 18 tandem
major
surface
protease (
msp) genes belonging to three classes (
mspL,
mspS,
mspC).
mspL and
mspS transcripts are differentially expressed during parasite growth. RNAs from
mspS genes predominate during stationary phase, the time when parasite virulence and GP63 expression are maximal. We hypothesized that the unique regions downstream of
mspS genes contain signals important for gene expression. The 2.8 kb region between tandem
mspS genes was found to contain an 882 bp open reading frame designated
mag. Copies of
mag were found downstream of all
mspS genes in the cluster.
mag hybridized faintly to bands on Northern blots and a fully processed
mag cDNA was identified in a promastigote cDNA library, providing evidence that
mag genes are expressed at low levels. Similar to
mspS RNAs, the abundance of
mag RNAs was greater in stationary phase than logarithmic phase organisms, although
mag RNAs were less abundant than
mspS RNAs throughout growth. Northern blots and enzyme assays of promastigotes containing plasmid constructs in which the
β-galactosidase gene was followed by sequences between
mspS coding regions, either with or without
mag and its downstream sequences, suggest these regions have several regulatory effects accounting for the growth-associated changes in
mspS expression.
Details
- Title: Subtitle
- Regulatory sequences and a novel gene in the msp (GP63) gene cluster of Leishmania chagasi
- Creators
- James J McCoy - Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USAJeffrey K Beetham - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USADiane E Ochs - Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USAJohn E Donelson - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USAMary E Wilson - Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Molecular and biochemical parasitology, Vol.95(2), pp.251-265
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0166-6851(98)00112-1
- PMID
- 9803417
- ISSN
- 0166-6851
- eISSN
- 1872-9428
- Grant note
- DOI: 10.13039/100000968, name: American Heart Association, award: T32 AI07511; DOI: 10.13039/100000002, name: National Institutes of Health, award: AI32135, AI40591, DK/AI52550
- Language
- English
- Date published
- 1998
- Academic Unit
- Microbiology and Immunology; International Programs; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984001117602771
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