Journal article
Relationship between dendrites of serotonin neurons and large midline vessels of the medulla
The FASEB journal, Vol.20(4), pp.A785-A785
03/2006
DOI: 10.1096/fasebj.20.4.A785-c
Abstract
Previous studies have demonstrated that medullary raphé neurons are closely related to midline blood vessels. Here, we characterized this relationship in further detail. First, using 10 μm fluorescent beads, we found that α‐smooth muscle actin is a reliable marker of arteries. Then, we transcardially perfused adult rats and obtained 20 um thick cryostat brainstem sections. With actin staining, we analyzed large blood vessels of at least 30 μm diameter in the raphe pallidus and ventral portion of the raphe obscurus and found that 86% of these vessels are arteries, while 14% are veins. Serotonergic neurons were found next to both arteries and veins. We perfused adult Pet1‐YFP positive mice transcardially, stained 20mμthick cryostat sections with an antibody against MAP2, a dendritic marker, and found that some serotonin neurons contact arteries with their dendrites. To determine the percentage of chemosensitive serotonergic raphé neurons, we exposed adult Pet1‐YFP mice to 10% CO2 for 1 hour. We perfused the animals transcardially and stained for cFos, and found that a subset of serotonergic neurons near arteries expressed this marker. These results indicate that some chemosensitive serotonin neurons have dendrites near arteries. The subset that is near veins could sense venous CO2, or may play a role distinct from those near arteries.
Details
- Title: Subtitle
- Relationship between dendrites of serotonin neurons and large midline vessels of the medulla
- Creators
- Carolin Isabel Dohle - Yale UniversityChristi J Wylie - Case Western Reserve UniversityEvan S Deneris - Case Western Reserve UniversityGeorge B Richerson - Yale University
- Resource Type
- Journal article
- Publication Details
- The FASEB journal, Vol.20(4), pp.A785-A785
- Publisher
- Federation of American Societies for Experimental Biology
- DOI
- 10.1096/fasebj.20.4.A785-c
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Number of pages
- 1
- Language
- English
- Date published
- 03/2006
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Neurosurgery
- Record Identifier
- 9984072062502771
Metrics
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