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Relationship of CD34+ cell dose to early and late hematopoiesis following autologous peripheral blood stem cell transplantation
Journal article   Peer reviewed

Relationship of CD34+ cell dose to early and late hematopoiesis following autologous peripheral blood stem cell transplantation

J. E KISS, W. B RYBKA, A WINKELSTEIN, M DEMAGALHAES-SILVERMAN, J LISTER, P D'ANDREA and E BALL
Bone marrow transplantation (Basingstoke), Vol.19(4), pp.303-310
1997
DOI: 10.1038/sj.bmt.1700671
PMID: 9051238

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Abstract

We evaluated early and late hematopoietic reconstitution in 27 patients with advanced lymphoma, Hodgkin's disease, and breast or ovarian cancer after treatment using high-dose/myeloablative conditioning regimens and autologous peripheral blood stem cell PBSC) transplantation. Eighteen patients (67%) received G-CSF 5 micrograms/kg/day following chemotherapy and nine (33%) were mobilized using G-CSF alone. Each patient had 7 x 10(8) mononuclear cells (MNC) per kg collected. G-CSF was administered post-PBSC infusion. While all patients showed prompt granulocyte recovery by day 14, platelet recovery failed to occur in our (15%) heavily pretreated patients with non-Hodgkin's lymphoma. Retrospective analysis in 17 patients revealed that the infused number of CD34 surface antigen-positive cells correlated with time to granulocyte (r = 0.59, P = 0.012) and platelet (r = 0.58, P = 0.021) recovery. Patients receiving the higher numbers of CD34+ cells had consistently better hematologic parameters at 11 times examined. At 180 days post-transplant, the median Hb level was 124 g/l vs 88 g/l (P = 0.004); platelet count was 202 x 10(9)/l vs 25 x 10(9)/l (P = 0.004); and neutrophil count was 3100 x 10(6)/l vs 1400 x 10(6)/l (P = 0.15). Hemoglobin strongly correlated with the CD34+ cell dose at 360 days (r = 0.90, P = 0.01). We conclude that graft CD34+ cell content appears to be an indicator of the quality of late as well as early hematopoietic function.
Biological and medical sciences Medical sciences Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Bone marrow, stem cells transplantation. Graft versus host reaction Transfusions. Complications. Transfusion reactions. Cell and gene therapy

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