Journal article
Relationship of rotational correlation time from EPR spectroscopy and protein-membrane interaction
Journal of membrane science, Vol.118(1), pp.133-139
1996
DOI: 10.1016/0376-7388(96)00070-1
Abstract
A study was carried out to determine if rotational correlation time of spin-labeled hen egg lysozyme (HEL) interacting with ultrafiltration membranes could be used to infer protein-membrane interaction. Polysulfone and cellulosic membranes, which have notably different adsorption properties, and membranes with varying pore sizes were used in this study. Based on this study, it was determined that the rotational correlation time does reflect variations in protein adsorption and pore plugging on membranes. The rotational correlation times for the highly adsorbent polysulfone (2.82 × 10
−8 s) were significantly higher than those obtained from proteins on cellulosic membranes (0.62 × 10
−8 s) and from those in solution (0.17 × 10
−8 s). Rotational correlation time was also increased due to steric hindrance associated with pore plugging, although it was not as significant as the adsorption effect. This study indicates that the rotational time constant can be used to infer the type of protein-membrane interaction.
Details
- Title: Subtitle
- Relationship of rotational correlation time from EPR spectroscopy and protein-membrane interaction
- Creators
- S.F. Oppenheim - University of IowaG.R. Buettner - University of IowaV.G.J. Rodgers - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of membrane science, Vol.118(1), pp.133-139
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0376-7388(96)00070-1
- ISSN
- 0376-7388
- eISSN
- 1873-3123
- Language
- English
- Date published
- 1996
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984313087802771
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