Journal article
Relationships between the Abundance and Expression of Functional Genes from Vinyl Chloride (VC)-Degrading Bacteria and Geochemical Parameters at VC-Contaminated Sites
Environmental science & technology, Vol.51(21), pp.12164-12174
11/07/2017
DOI: 10.1021/acs.est.7b03521
PMID: 28981261
Abstract
Bioremediation of vinyl chloride (VC) contamination in groundwater could be mediated by three major bacterial guilds: anaerobic VC-dechlorinators, methanotrophs, and ethene-oxidizing bacteria (etheneotrophs) via metabolic or cometabolic pathways. We collected 95 groundwater samples across 6 chlorinated ethene-contaminated sites and searched for relationships among VC biodegradation gene abundance and expression and site geochemical parameters (e.g., VC concentrations). Functional genes from the three major VC-degrading bacterial guilds were present in 99% and expressed in 59% of the samples. Etheneotroph and methanotroph functional gene abundances ranged from 10
to 10
genes per liter of groundwater among the samples with VC reductive dehalogenase gene (bvcA and vcrA) abundances reaching 10
genes per liter of groundwater. Etheneotroph functional genes (etnC and etnE) and VC reductive dehalogenase genes (bvcA and vcrA) were strongly related to VC concentrations (p < 0.001). Methanotroph functional genes (mmoX and pmoA) were not related to VC concentration (p > 0.05). Samples from sites with bulk VC attenuation rates >0.08 year
contained higher levels of etheneotroph and anaerobic VC-dechlorinator functional genes and transcripts than those with bulk VC attenuation rates <0.004 year
. We conclude that both etheneotrophs and anaerobic VC-dechlorinators have the potential to simultaneously contribute to VC biodegradation at these sites.
Details
- Title: Subtitle
- Relationships between the Abundance and Expression of Functional Genes from Vinyl Chloride (VC)-Degrading Bacteria and Geochemical Parameters at VC-Contaminated Sites
- Creators
- Yi LiangXikun LiuMichael A Singletary - NAVFAC Southeast, EV3 Environmental Restoration Building 135, Naval Air Station Jacksonville, Florida 32508, United StatesKai WangTimothy E Mattes
- Resource Type
- Journal article
- Publication Details
- Environmental science & technology, Vol.51(21), pp.12164-12174
- Publisher
- United States
- DOI
- 10.1021/acs.est.7b03521
- PMID
- 28981261
- ISSN
- 0013-936X
- eISSN
- 1520-5851
- Grant note
- DOI: 10.13039/100000005, name: U.S. Department of Defense, award: ER-201425
- Language
- English
- Date published
- 11/07/2017
- Academic Unit
- Civil and Environmental Engineering; Biostatistics
- Record Identifier
- 9983997351102771
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