Journal article
Relative proportion and distribution of chemosensitive serotonergic neurons in medullary raphé in situ
The FASEB journal, Vol.25, pp.847.4-847.4
04/2011
DOI: 10.1096/fasebj.25.1_supplement.847.4
Abstract
The identity and distribution of brainstem chemosensitive neurons are enigmatic. Serotonin synthesizing (5HT) neurons, cultured from raphé, are intrinsically sensitive to elevations in CO2 and/or acidosis. We have recently identified that chemosensitive 5HT neurons also occur in a more intact system, the unanesthetized decerebrate juvenile rat in situ perfused brainstem preparation. The present study tests the hypothesis that medullary raphé neurons are a heterogeneous population, consisting of both CO2/pH sensitive and insensitive neurons. Extracellular recordings demonstrate chemosensitive and insensitive neurons, while juxtacellular labeling of recorded neurons and subsequent immunohistochemistry identify neurotransmitter phenotypes of electrophysiologically characterized cells. Here we show that approximately 25% of recorded and immunohistochemically characterized 5HT neurons are chemosensitive, supporting the conclusion that raphé chemosensitive neurons occur as a substantial subset within a heterogeneous population. Assessment of the distributions of sensitive and insensitive cells across the raphé is ongoing. Supported by NIH 2U54NS041069‐06A1.
Details
- Title: Subtitle
- Relative proportion and distribution of chemosensitive serotonergic neurons in medullary raphé in situ
- Creators
- Kimberly Erin Iceman - University of Alaska FairbanksGeorge B Richerson - University of IowaMichael Bruce Harris - University of Alaska Fairbanks
- Resource Type
- Journal article
- Publication Details
- The FASEB journal, Vol.25, pp.847.4-847.4
- Publisher
- Federation of American Societies for Experimental Biology
- DOI
- 10.1096/fasebj.25.1_supplement.847.4
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Number of pages
- 1
- Grant note
- NIH (2U54NS041069‐06A1)
- Language
- English
- Date published
- 04/2011
- Academic Unit
- Neurosurgery; Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
- Record Identifier
- 9984072066602771
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