Relative release of interleukin-1 beta and interleukin-1 receptor antagonist by alveolar macrophages. A study in asbestos-induced lung disease, sarcoidosis, and idiopathic pulmonary fibrosis

Joel N Kline; David A Schwartz; Martha M Monick; Connie S. Floerchinger; Gary W Hunninghake

doi:10.1378/chest.104.1.47

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Relative release of interleukin-1 beta and interleukin-1 receptor antagonist by alveolar macrophages. A study in asbestos-induced lung disease, sarcoidosis, and idiopathic pulmonary fibrosis

Journal article

Peer reviewed

Relative release of interleukin-1 beta and interleukin-1 receptor antagonist by alveolar macrophages. A study in asbestos-induced lung disease, sarcoidosis, and idiopathic pulmonary fibrosis

Joel N Kline, David A Schwartz, Martha M Monick, Connie S. Floerchinger and Gary W Hunninghake

Chest, Vol.104(1), pp.47-53

07/1993

DOI: 10.1378/chest.104.1.47

PMID: 8325116

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Abstract

We examined the influence of untreated interstitial lung disease (ILD) on the in vitro release of interleukin-1 beta (IL-1 beta) and interleukin-1 receptor antagonist (IL-Ira) from alveolar macrophages (AM); AM were harvested from normal volunteers, ILD patients, and patients with asbestos-related pleural disease but no ILD. AM were cultured for 24 h and assays for IL-1 beta and IL-1ra were done using sensitive and specific enzyme-linked immunosorbent assay. A greater amount of IL-1 beta was detected in AM supernatants from asbestosis, sarcoidosis, and IPF patients than in those from normal subjects. The IL-1 beta:IL-1ra ratio (IL-1 beta activity index [IL-1AI]) was significantly lower in supernatants of normal macrophages compared with macrophage supernatants from individuals with ILD. The IL-1AI correlated with bronchoalveolar lavage cellularity, a marker of disease activity. Current smoking was associated with lower IL-1 beta and IL-1ra release in ILD. The IL-1AI is a convenient method for comparison of IL-1 beta activity between patient populations.

Leukocytes - pathology

Lung Diseases - metabolism

Humans

Middle Aged

Male

Asbestosis - metabolism

Bronchoalveolar Lavage Fluid - pathology

Adult

Female

Pulmonary Fibrosis - metabolism

Interleukin-1 - metabolism

Interleukin-1 - analysis

Cells, Cultured

Pulmonary Fibrosis - pathology

Receptors, Interleukin-1 - analysis

Pleural Diseases - metabolism

Smoking - metabolism

Fibrosis

Smoking - pathology

Aged

Lung Diseases - pathology

Macrophages, Alveolar - metabolism

Receptors, Interleukin-1 - antagonists & inhibitors

Leukocytes - metabolism

Asbestosis - pathology

Sarcoidosis - metabolism

Pleural Diseases - pathology

Sarcoidosis - pathology

Details

Title: Subtitle: Relative release of interleukin-1 beta and interleukin-1 receptor antagonist by alveolar macrophages. A study in asbestos-induced lung disease, sarcoidosis, and idiopathic pulmonary fibrosis
Creators: Joel N Kline - Department of Internal Medicine, Veteran's Affairs Medical Center, Iowa City
David A Schwartz
Martha M Monick
Connie S. Floerchinger
Gary W Hunninghake
Resource Type: Journal article
Publication Details: Chest, Vol.104(1), pp.47-53
DOI: 10.1378/chest.104.1.47
PMID: 8325116
ISSN: 0012-3692
eISSN: 1931-3543
Grant note: NRSA HL 07638-07 / NHLBI NIH HHS NIEHS CIA ES 00203 / NIEHS NIH HHS NHLBI SCOR HL 37121 / NHLBI NIH HHS
Language: English
Date published: 07/1993
Academic Unit: Pulmonary, Critical Care, and Occupational Medicine; Occupational and Environmental Health; Internal Medicine
Record Identifier: 9984094481602771

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