Journal article
Reliability of 7T 1H‐MRS measured human prefrontal cortex glutamate, glutamine, and glutathione signals using an adapted echo time optimized PRESS sequence: A between‐ and within‐sessions investigation
Journal of magnetic resonance imaging, Vol.43(1), pp.88-98
01/2016
DOI: 10.1002/jmri.24970
PMCID: PMC4671833
PMID: 26059603
Abstract
Purpose
To ascertain the mechanisms of neuropsychiatric illnesses and their treatment, accurate and reliable imaging techniques are required; proton magnetic resonance spectroscopy (1H‐MRS) can noninvasively measure glutamatergic function. Evidence suggests that aberrant glutamatergic signaling plays a role in numerous psychopathologies. Until recently, overlapping glutamatergic signals (glutamate, glutamine, and glutathione) could not easily be separated. However, the advent of novel pulse sequences and higher field magnetic resonance imaging (MRI) allows more precise resolution of overlapping glutamatergic signals, although the question of signal reliability remains undetermined.
Materials and Methods
At 7T MR, we acquired 1H‐MRS data from the medial pregenual anterior cingulate cortex of healthy volunteers (n = 26) twice on two separate days. An adapted echo time optimized point‐resolved spectroscopy sequence, modified with the addition of a J‐suppression pulse to attenuate N‐acetyl‐aspartate multiplet signals at 2.49 ppm, was used to excite and acquire the spectra. In‐house software was used to model glutamate, glutamine, and glutathione, among other metabolites, referenced to creatine. Intraclass correlation coefficients (ICCs) were computed for within‐ and between‐session measurements.
Results
Within‐session measurements of glutamate, glutamine, and glutathione were on average reliable (ICCs ≥0.7). As anticipated, ICCs for between‐session values of glutamate, glutamine, and glutathione were slightly lower but nevertheless reliable (ICC >0.62). A negative correlation was observed between glutathione concentration and age (r(24) = –0.37; P < 0.05), and a gender effect was noted on glutamine and glutathione.
Conclusion
The adapted sequence provides good reliability to measure glutamate, glutamine, and glutathione signals. J. MAGN. RESON. IMAGING 2016;43:88–98.
Details
- Title: Subtitle
- Reliability of 7T 1H‐MRS measured human prefrontal cortex glutamate, glutamine, and glutathione signals using an adapted echo time optimized PRESS sequence: A between‐ and within‐sessions investigation
- Creators
- Níall Lally - University College LondonLi An - National Institutes of HealthDipavo Banerjee - National Institutes of HealthMark J Niciu - National Institutes of HealthDavid A Luckenbaugh - National Institutes of HealthErica M Richards - National Institutes of HealthJonathan P Roiser - University College LondonJun Shen - National Institutes of HealthCarlos A Zarate - National Institutes of HealthAllison C Nugent - National Institutes of Health
- Resource Type
- Journal article
- Publication Details
- Journal of magnetic resonance imaging, Vol.43(1), pp.88-98
- DOI
- 10.1002/jmri.24970
- PMID
- 26059603
- PMCID
- PMC4671833
- NLM abbreviation
- J Magn Reson Imaging
- ISSN
- 1053-1807
- eISSN
- 1522-2586
- Number of pages
- 11
- Grant note
- Wellcome Trust‐National Institutes of Health joint PhD studentship (WT095465) NARSAD Independent Investigator Intramural Research Program of the National Institute of Mental Health, National Institutes of Health (IRP‐NIMH‐NIH; NCT00397111; protocol number 07‐M‐0021) Brain & Behavior Mood Disorders Research Award
- Language
- English
- Date published
- 01/2016
- Academic Unit
- Psychiatry; Iowa Neuroscience Institute
- Record Identifier
- 9984003445102771
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