Journal article
Reliable and reusable whole polypropylene plastic microfluidic devices for a rapid, low-cost antimicrobial susceptibility test
Lab on a chip, Vol.19(17), pp.2915-2924
08/20/2019
DOI: 10.1039/C9LC00502A
PMID: 31369010
Abstract
Using an antimicrobial susceptibility test (AST) as an example, this work demonstrates a practical method to fabricate microfluidic chips entirely from polypropylene (PP) and the benefits for potential commercial use. Primarily caused by the misuse and abuse of antibiotics, antimicrobial resistance (AMR) is a major threat to modern medicine. The AST is a promising technique to help with the optimal use of antibiotics for reducing AMR. However, current phenotypic ASTs suffer from long turnaround time, while genotypic ASTs suffer from low reliability, and both are unaffordable for routine use. New microfluidics based AST methods are rapid but still unreliable as well as costly due to the PDMS chip material. Herein, we demonstrate a convenient method to fabricate whole PP microfluidic chips with high resolution and fidelity. Unlike PDMS chips, the whole PP chips showed better reliability due to their inertness; they are solvent-compatible and can be conveniently reused and recycled, which largely decreases the cost, and are environmentally friendly. We specially designed 3D chambers that allow for quick cell loading without valving/liquid exchange; this new hydrodynamic design satisfies the shear stress requirement for on-chip bacterial culture, which, compared to reported designs for similar purposes, allows for a simpler, more rapid, and high-throughput operation. Our system allows for reliable tracking of individual cells and acquisition of AST results within 1–3 hours, which is among the group of fastest phenotypic methods. The PP chips are more reliable and affordable than PDMS chips, providing a practical solution to improve current culture-based AST and benefiting the fight against AMR through helping doctors prescribe effective, narrow-spectrum antibiotics; they will also be broadly useful for other applications wherein a reliable, solvent-resistant, anti-fouling, and affordable microfluidic chip is needed.
Details
- Title: Subtitle
- Reliable and reusable whole polypropylene plastic microfluidic devices for a rapid, low-cost antimicrobial susceptibility test
- Creators
- Han Sun - Hong Kong Baptist UniversityChiu-Wing Chan - Hong Kong Baptist UniversityYisu Wang - Hong Kong Baptist UniversityXiao Yao - Hong Kong Baptist UniversityXuan Mu - Tufts UniversityXuedong Lu - Sun Yat-sen UniversityJianhua Zhou - Sun Yat-sen UniversityZongwei Cai - Hong Kong Baptist UniversityKangning Ren - Hong Kong Baptist University
- Resource Type
- Journal article
- Publication Details
- Lab on a chip, Vol.19(17), pp.2915-2924
- DOI
- 10.1039/C9LC00502A
- PMID
- 31369010
- ISSN
- 1473-0197
- eISSN
- 1473-0189
- Grant note
- DOI: 10.13039/501100001747, name: Hong Kong Baptist University, award: RC-IRMS/16-17/03, FRG2/17-18/102; DOI: 10.13039/501100001809, name: National Natural Science Foundation of China, award: 21505110, 51773173; DOI: 10.13039/501100002920, name: Research Grants Council, University Grants Committee, award: 22200515; DOI: 10.13039/501100007156, name: Innovation and Technology Commission - Hong Kong, award: ITS/260/16FX, HKBU SKL-CRF, SKLP_1718_P01
- Language
- English
- Date published
- 08/20/2019
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering
- Record Identifier
- 9984276454102771
Metrics
6 Record Views