Removal of Mg2+ inhibition of cardiac ryanodine receptor by palmitoyl coenzyme A

Timothy Connelly; Christopher Ahern; Manana Sukhareva; Roberto Coronado

doi:10.1016/0014-5793(94)00969-4

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Removal of Mg2+ inhibition of cardiac ryanodine receptor by palmitoyl coenzyme A

Journal article

Open access

Peer reviewed

Removal of Mg2+ inhibition of cardiac ryanodine receptor by palmitoyl coenzyme A

Timothy Connelly, Christopher Ahern, Manana Sukhareva and Roberto Coronado

FEBS letters, Vol.352(3), pp.285-290

10/03/1994

DOI: 10.1016/0014-5793(94)00969-4

PMID: 7925988

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Abstract

45Ca2+ fluxes and planar bilayer recordings indicated that the fatty acid metabolise palmitoyl coenzyme A, but not free coenzyme A or palmitic acid, stimulated the cardiac ryanodine receptor channel of pig heart sarcoplasmic reticulum. Palmitoyl CoA reactivated channels inhibited by concentrations of cytoplasmic free Mg2+ in the physiological range. Reactivation by palmitoyl CoA in the presence of Mg2+ was stimulated by myoplasmic free Ca2+ in the micromolar range. Acyl coenzyme A derivatives may be utilized by cardiac muscle cells to compensate for the severe Mg2+ inhibition of ryanodine receptors which would otherwise leave Ca2+ stores unresponsive to Ca2+ and to other cytosolic ligands involved in signal transduction.

Ca2+ release channel

Sarcoplasmic reticulum

MES, (2-[N-Morpholino] ethanesulfonic acid)

Ryanodine receptor

Tris, (Tris[hydroxymethyl] aminomethane)

EGTA, (ethyleneglygol-bis(β-aminoethyl ether)-N,N,N′,N′-tetra acetic acid)

Fatty acid metabolite

Details

Title: Subtitle: Removal of Mg2+ inhibition of cardiac ryanodine receptor by palmitoyl coenzyme A
Creators: Timothy Connelly
Christopher Ahern
Manana Sukhareva
Roberto Coronado
Resource Type: Journal article
Publication Details: FEBS letters, Vol.352(3), pp.285-290
DOI: 10.1016/0014-5793(94)00969-4
PMID: 7925988
ISSN: 0014-5793
eISSN: 1873-3468
Number of pages: 6
Language: English
Date published: 10/03/1994
Academic Unit: Molecular Physiology and Biophysics; Iowa Neuroscience Institute
Record Identifier: 9984070976202771

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