Journal article
Removal of potentially confounding phenotypes from a Siamese-derived feline glaucoma breeding colony
Comparative medicine, Vol.61(3), pp.251-257
06/2011
PMCID: PMC3123758
PMID: 21819695
Abstract
Feline breeding colonies face genetic constraints involving founder effects. A Siamese-founded colony used to study primary congenital glaucoma displayed coat colors additional to the Siamese coat. Genes affecting pigment can exhibit pleiotropy on ocular development and function. To remove potentially confounding phenotypes from our colony, we documented the source and frequency of the Siamese allele at the gene for tyrosinase (TYR), the dilution allele at melanophilin (MLPH), and the brown allele at tyrosinase-related protein 1 (TYRP1). We used PCR-RFLP diagnostics to genotype cats in our colony for the published alleles. A commercially acquired phenotypically normal tom was the source of the dilute allele. A founding Siamese queen was the source of the brown allele. Founders also were blood-typed and screened for disease-associated alleles segregating in Siamese cats at 3 loci (ASB, GLB1, and CEP290). Siamese founders were normal at all loci except ASB, at which both animals carried the hypomorpic allele. Current stock is being managed to limit production of glaucomatous cats with brown, dilute, or Siamese phenotypes or homozygosity for the ASB hypomorphic allele. Genotyping will aid in the elimination of these alleles. The clinical effect of these phenotypes and alleles on the glaucoma phenotype is uncertain, but their elimination will remove potentially confounding effects. In conclusion, when founding a colony, stock should be selected or screened to limit potentially confounding phenotypes. When studying the immune, nervous, and visual systems, screening stock for alleles known to be associated with coat color may be warranted.
Details
- Title: Subtitle
- Removal of potentially confounding phenotypes from a Siamese-derived feline glaucoma breeding colony
- Creators
- Michelle M Rutz-Mendicino - Department of Animal Science and the Center for Integrated Animal Genomics, Iowa State University, Ames, Iowa, USAElizabeth M SnellaJackie K JensBarbara GandolfiSteven A CarlsonMarkus H KuehnGillian J McLellanN Matthew Ellinwood
- Resource Type
- Journal article
- Publication Details
- Comparative medicine, Vol.61(3), pp.251-257
- Publisher
- United States
- PMID
- 21819695
- PMCID
- PMC3123758
- ISSN
- 1532-0820
- Grant note
- P30 EY016665 / NEI NIH HHS P30EY016665 / NEI NIH HHS 5K08EY018609 / NEI NIH HHS K08 EY018609 / NEI NIH HHS
- Language
- English
- Date published
- 06/2011
- Academic Unit
- Ophthalmology and Visual Sciences
- Record Identifier
- 9983979930702771
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