Removal of sodium and potassium adducts using a matrix additive during matrix-associated laser desorption/ionization time-of-flight mass spectrometric analysis of peptides

John F Leite; Mahbod R Hajivandi; Thomas Diller; R Marshall Pope

doi:10.1002/rcm.1711

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Removal of sodium and potassium adducts using a matrix additive during matrix-associated laser desorption/ionization time-of-flight mass spectrometric analysis of peptides

Journal article

Peer reviewed

Removal of sodium and potassium adducts using a matrix additive during matrix-associated laser desorption/ionization time-of-flight mass spectrometric analysis of peptides

John F Leite, Mahbod R Hajivandi, Thomas Diller and R Marshall Pope

Rapid communications in mass spectrometry, Vol.18(23), pp.2953-2959

01/01/2004

DOI: 10.1002/rcm.1711

PMID: 15536629

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Abstract

Monovalent cations often associate with peptides and proteins under mass spectrometry (MS) conditions, resulting in a discernable, but often misleading, adduct cluster pattern. These adduct cluster peaks reduce the signal intensity of specific peptide species by splitting the ion population into multiple mass peaks, suppressing the ionization of neighboring low-abundance peaks, and interfering with identification of post-translational modifications. Further, monovalent contaminants tend to form a distribution of matrix cluster peaks in matrix-associated laser desorption/ionization time-of-flight (MALDI-TOF) spectra causing interference and suppression in the mass range below 1400 Da. The most common method for reduction or elimination of adduct clusters is solid-phase extraction via a pipette tip or spin column, which often leads to loss of low-abundance peptide components. In this study we describe the use of a commercially available surfactant blend that markedly reduces the adduction of monovalent cations during peptide analysis by MALDI-TOFMS.

Animals

Cattle

Peptides - analysis

Potassium - chemistry

Serum Albumin, Bovine - chemistry

Sodium - chemistry

Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods

Surface-Active Agents

Details

Title: Subtitle: Removal of sodium and potassium adducts using a matrix additive during matrix-associated laser desorption/ionization time-of-flight mass spectrometric analysis of peptides
Creators: John F Leite - CrossLife Technologies
Mahbod R Hajivandi - CrossLife Technologies
Thomas Diller - CrossLife Technologies
R Marshall Pope - CrossLife Technologies
Resource Type: Journal article
Publication Details: Rapid communications in mass spectrometry, Vol.18(23), pp.2953-2959
DOI: 10.1002/rcm.1711
PMID: 15536629
ISSN: 0951-4198
eISSN: 1097-0231
Language: English
Date published: 01/01/2004
Academic Unit: Medicine Administration
Record Identifier: 9984627340802771

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