Reovirus preferentially infects the basolateral surface and is released from the apical surface of polarized human respiratory epithelial cells

Katherine J D A Excoffon; Kristen M Guglielmi; J Denise Wetzel; Nicholas D Gansemer; Jacquelyn A Campbell; Terence S Dermody; Joseph Zabner

doi:10.1086/529515

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Reovirus preferentially infects the basolateral surface and is released from the apical surface of polarized human respiratory epithelial cells

Journal article

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Reovirus preferentially infects the basolateral surface and is released from the apical surface of polarized human respiratory epithelial cells

Katherine J D A Excoffon, Kristen M Guglielmi, J Denise Wetzel, Nicholas D Gansemer, Jacquelyn A Campbell, Terence S Dermody and Joseph Zabner

The Journal of infectious diseases, Vol.197(8), pp.1189-1197

04/15/2008

DOI: 10.1086/529515

PMCID: PMC2736797

PMID: 18419529

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Abstract

Mammalian reoviruses infect respiratory and gastrointestinal epithelia and cause disease in neonates. Junctional adhesion molecule-A (JAM-A) is a serotype-independent receptor for reovirus. JAM-A localizes to tight junctions and contributes to paracellular permeability in polarized epithelia. To investigate the mechanisms of reovirus infection of polarized epithelial cells, we assessed reovirus replication, release, and spread after apical and basolateral adsorption to primary human airway epithelial cultures. Reovirus infection of human airway epithelia was more efficient after adsorption to the basolateral surface than after adsorption to the apical surface, and it was dependent on JAM-A. Reovirus binding to carbohydrate coreceptor sialic acid inhibited apical infection, which was partially ameliorated by treatment of the cultures with neuraminidase. Despite the preference for basolateral infection, reovirus was released from the apical surface of respiratory epithelia and did not disrupt tight junctions. These results establish the existence of an infectious circuit for reovirus in polarized human respiratory epithelial cells.

Immunohistochemistry

Tight Junctions - metabolism

Respiratory Mucosa - cytology

Reassortant Viruses - pathogenicity

Humans

Tight Junctions - virology

L Cells (Cell Line)

Receptors, Cell Surface

Respiratory Mucosa - virology

Virus Shedding

Electric Impedance

Cell Adhesion Molecules - metabolism

Reoviridae Infections - virology

Immunoglobulins - metabolism

Neuraminidase - pharmacology

Microscopy, Confocal

Animals

Orthoreovirus, Mammalian - pathogenicity

Cell Polarity - physiology

Mice

Respiratory Mucosa - metabolism

Respiratory Tract Infections - virology

Orthoreovirus, Mammalian - metabolism

Details

Title: Subtitle: Reovirus preferentially infects the basolateral surface and is released from the apical surface of polarized human respiratory epithelial cells
Creators: Katherine J D A Excoffon - Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
Kristen M Guglielmi
J Denise Wetzel
Nicholas D Gansemer
Jacquelyn A Campbell
Terence S Dermody
Joseph Zabner
Resource Type: Journal article
Publication Details: The Journal of infectious diseases, Vol.197(8), pp.1189-1197
DOI: 10.1086/529515
PMID: 18419529
PMCID: PMC2736797
ISSN: 0022-1899
eISSN: 1537-6613
Grant note: R37 AI038296-12 / NIAID NIH HHS R37 AI038296-13 / NIAID NIH HHS T32 CA09385 / NCI NIH HHS P01 HL51670 / NHLBI NIH HHS P30 DK020593 / NIDDK NIH HHS CA68485 / NCI NIH HHS DK20593 / NIDDK NIH HHS P60 DK020593 / NIDDK NIH HHS T32 GM008554 / NIGMS NIH HHS T32 GM08554 / NIGMS NIH HHS P30 CA068485 / NCI NIH HHS T32 CA009385 / NCI NIH HHS R37 AI38296 / NIAID NIH HHS P01 HL051670 / NHLBI NIH HHS R37 AI038296 / NIAID NIH HHS P30 DK054759 / NIDDK NIH HHS DK54759 / NIDDK NIH HHS
Language: English
Date published: 04/15/2008
Academic Unit: Pulmonary, Critical Care, and Occupational Medicine; Internal Medicine
Record Identifier: 9984094336302771

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