Repair of DNA Breaks by Break-Induced Replication

Z W Kockler; B Osia; R Lee; K Musmaker; A Malkova

doi:10.1146/annurev-biochem-081420-095551

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Repair of DNA Breaks by Break-Induced Replication

Journal article

Open access

Peer reviewed

Repair of DNA Breaks by Break-Induced Replication

Z W Kockler, B Osia, R Lee, K Musmaker and A Malkova

Annual review of biochemistry, Vol.90(1), pp.165-191

06/20/2021

DOI: 10.1146/annurev-biochem-081420-095551

PMCID: PMC9629446

PMID: 33792375

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https://doi.org/10.1146/annurev-biochem-081420-095551View

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Abstract

Double-strand DNA breaks (DSBs) are the most lethal type of DNA damage, making DSB repair critical for cell survival. However, some DSB repair pathways are mutagenic and promote genome rearrangements, leading to genome destabilization. One such pathway is break-induced replication (BIR), which repairs primarily one-ended DSBs, similar to those formed by collapsed replication forks or telomere erosion. BIR is initiated by the invasion of a broken DNA end into a homologous template, synthesizes new DNA within the context of a migrating bubble, and is associated with conservative inheritance of new genetic material. This mode of synthesis is responsible for a high level of genetic instability associated with BIR. Eukaryotic BIR was initially investigated in yeast, but now it is also actively studied in mammalian systems. Additionally, a significant breakthrough has been made regarding the role of microhomology-mediated BIR in the formation of complex genomic rearrangements that underly various human pathologies.

DNA Repair

DNA Replication

Mutation

Animals

DNA Breaks, Double-Stranded

DNA End-Joining Repair

Humans

Mammals - genetics

Telomere Homeostasis - genetics

Yeasts - genetics

Details

Title: Subtitle: Repair of DNA Breaks by Break-Induced Replication
Creators: Z W Kockler - Department of Biology, University of Iowa, Iowa City, Iowa 52242, USA; email: anna-malkova@uiowa.edu
B Osia - Department of Biology, University of Iowa, Iowa City, Iowa 52242, USA; email: anna-malkova@uiowa.edu
R Lee - Department of Biology, University of Iowa, Iowa City, Iowa 52242, USA; email: anna-malkova@uiowa.edu
K Musmaker - Department of Biology, University of Iowa, Iowa City, Iowa 52242, USA; email: anna-malkova@uiowa.edu
A Malkova - Department of Biology, University of Iowa, Iowa City, Iowa 52242, USA; email: anna-malkova@uiowa.edu
Resource Type: Journal article
Publication Details: Annual review of biochemistry, Vol.90(1), pp.165-191
DOI: 10.1146/annurev-biochem-081420-095551
PMID: 33792375
PMCID: PMC9629446
NLM abbreviation: Annu Rev Biochem
ISSN: 0066-4154
eISSN: 1545-4509
Grant note: R35 GM127006 / NIGMS NIH HHS
Language: English
Date published: 06/20/2021
Academic Unit: Biology
Record Identifier: 9984217528902771

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