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Repeated nitrogen starvation doesn't affect lipid productivity of Chlorococcum littorale
Journal article   Open access   Peer reviewed

Repeated nitrogen starvation doesn't affect lipid productivity of Chlorococcum littorale

Iago Teles Dominguez Cabanelas, Dorinde M. M. Kleinegris, Rene H. Wijffels and Maria J. Barbosa
Bioresource technology, Vol.219, pp.576-582
11/01/2016
DOI: 10.1016/j.biortech.2016.08.009
PMID: 27540634
url
https://doi.org/10.1016/j.biortech.2016.08.009View
Published (Version of record) Open Access

Abstract

In the present work we wanted to know what happens during time to biomass and lipid productivities of Chlorococcum littorale repeatedly subjected to N-starvation. Experiments were done using repeated cycles of batch-wise N run-out (after 2 days N = 0). Two different cycles were used: repeated short-starvation (6 days of N = 0) over a total period of 72 days and repeated long-starvation (13 days of N = 0) over a total period of 75 days. Batches (using fresh inocula) were done separately as control. Shorter and longer periods of starvation showed no differences in biomass productivities and PSII quantum yield evolution. The repeated short-starvation-batches showed the same lipid productivities as the control short-starvation batches. Most importantly, the biomass lipid content was the same between control and repeated-batches. Altogether, the results point to C. littorale as a resilient and stable strain, with potential to be used under semi continuous cultivation. (C) 2016 The Authors. Published by Elsevier Ltd.
Agricultural Engineering Agriculture Biotechnology & Applied Microbiology Energy & Fuels Life Sciences & Biomedicine Science & Technology Technology

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