Journal article
Repetitive Antigen Stimulation Induces Stepwise Transcriptome Diversification but Preserves a Core Signature of Memory CD8+ T Cell Differentiation
Immunity (Cambridge, Mass.), Vol.33(1), pp.128-140
07/23/2010
DOI: 10.1016/j.immuni.2010.06.014
PMCID: PMC2912220
PMID: 20619696
Abstract
Repetitive antigen stimulation by prime-boost vaccination or pathogen reencounter increases memory CD8+ T cell numbers, but the impact on memory CD8+ T cell differentiation is unknown. Here we showed that repetitive antigen stimulations induced accumulation of memory CD8+ T cells with uniform effector memory characteristics. However, genome-wide microarray analyses revealed that each additional antigen challenge resulted in the differential regulation of several hundred new genes in the ensuing memory CD8+ T cell populations and, therefore, in stepwise diversification of CD8+ T cell transcriptomes. Thus, primary and repeatedly stimulated (secondary, tertiary, and quaternary) memory CD8+ T cells differed substantially in their molecular signature while sharing expression of a small group of genes and biological pathways, which may constitute a core signature of memory differentiation. These results reveal the complex regulation of memory CD8+ T cell differentiation and identify potential new molecular targets to dissect the function of memory cells generated by repeated antigen stimulation.
► Repeated antigen stimulation alters memory CD8+ T cell phenotype and function ► Repeatedly stimulated Tm cells preserve a core signature of memory differentiation ► Tm transcriptome diversity increases by hundreds of new genes with each Ag encounter ► Repeatedly stimulated Tm populations exhibit unique gene-expression signatures
Details
- Title: Subtitle
- Repetitive Antigen Stimulation Induces Stepwise Transcriptome Diversification but Preserves a Core Signature of Memory CD8+ T Cell Differentiation
- Creators
- Thomas C Wirth - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USAHai-Hui Xue - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USADeepa Rai - Department of Pathology, University of Iowa, Iowa City, IA 52242, USAJaime T Sabel - Department of Pathology, University of Iowa, Iowa City, IA 52242, USATom Bair - DNA Facility, University of Iowa, Iowa City, IA 52242, USAJohn T Harty - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USAVladimir P Badovinac - Department of Pathology, University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Immunity (Cambridge, Mass.), Vol.33(1), pp.128-140
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.immuni.2010.06.014
- PMID
- 20619696
- PMCID
- PMC2912220
- ISSN
- 1074-7613
- eISSN
- 1097-4180
- Language
- English
- Date published
- 07/23/2010
- Academic Unit
- Microbiology and Immunology; Pathology
- Record Identifier
- 9984047610702771
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