Logo image
Back
Replication Protein A Is a Component of a Complex That Binds the Human Metallothionein IIA Gene Transcription Start Site
Journal article   Open access   Peer reviewed

Replication Protein A Is a Component of a Complex That Binds the Human Metallothionein IIA Gene Transcription Start Site

Chih-Min Tang, Alan E Tomkinson, William S Lane, Marc S Wold and Edward Seto
The Journal of biological chemistry, Vol.271(35), pp.21637-21644
08/30/1996
DOI: 10.1074/jbc.271.35.21637
PMID: 8702952
url
https://doi.org/10.1074/jbc.271.35.21637View
Published (Version of record) Open Access

Abstract

Previous studies revealed that sequences surrounding the initiation sites in many mammalian and viral gene promoters, called initiator (Inr) elements, may be essential for promoter strength and for determining the actual transcription start sites. DNA sequences in the vicinity of the human metallothionein IIA (hMTIIA) gene transcription start site share homology with some of the previously identified Inr elements. However, in the present study we have found by in vitro transcription assays that the hMTIIA promoter does not contain a typical Inr. Electrophoretic mobility shift assays identified several DNA-protein complexes at the hMTIIA gene transcription start site. A partially purified protein fraction containing replication protein A (RPA) binds to the hMTIIA gene transcription start site and represses transcription from the hMTIIA promoter in vitro. In addition, overexpression of the human 70-kDa RPA-1 protein represses transcription of a reporter gene controlled by the hMTIIA promoter in vivo. These findings suggest that hMTIIA transcription initiation is controlled by a mechanism different from most mammalian and viral promoters and that the previously identified RPA may also be involved in transcription regulation.

Details

Metrics

27 Record Views
18 Times Cited - Web of Science
Logo image