Journal article
Replication Protein A phosphorylation and the cellular response to DNA damage
DNA Repair, Vol.3(8), pp.1015-1024
2004
DOI: 10.1016/j.dnarep.2004.03.028
PMID: 15279788
Abstract
Defects in cellular DNA metabolism have a direct role in many human disease processes. Impaired responses to DNA damage and basal DNA repair have been implicated as causal factors in diseases with DNA instability like cancer, Fragile X and Huntington’s. Replication protein A (RPA) is essential for multiple processes in DNA metabolism including DNA replication, recombination and DNA repair pathways (including nucleotide excision, base excision and double-strand break repair). RPA is a single-stranded DNA-binding protein composed of subunits of 70-, 32- and 14-kDa. RPA binds ssDNA with high affinity and interacts specifically with multiple proteins. Cellular DNA damage causes the N-terminus of the 32-kDa subunit of human RPA to become hyper-phosphorylated. Current data indicates that hyper-phosphorylation causes a change in RPA conformation that down-regulates activity in DNA replication but does not affect DNA repair processes. This suggests that the role of RPA phosphorylation in the cellular response to DNA damage is to help regulate DNA metabolism and promote DNA repair.
Details
- Title: Subtitle
- Replication Protein A phosphorylation and the cellular response to DNA damage
- Creators
- Sara K BinzAnne M SheehanMarc S Wold
- Resource Type
- Journal article
- Publication Details
- DNA Repair, Vol.3(8), pp.1015-1024
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.dnarep.2004.03.028
- PMID
- 15279788
- ISSN
- 1568-7864
- eISSN
- 1568-7856
- Language
- English
- Date published
- 2004
- Academic Unit
- Radiation Oncology; Biochemistry and Molecular Biology
- Record Identifier
- 9984024513902771
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