Journal article
Replication of Chronic Abnormal Cartilage Loading by Medial Meniscus Destabilization for Modeling Osteoarthritis in the Rabbit Knee In Vivo
Journal of orthopaedic research, Vol.31(10), pp.1555-1560
10/2013
DOI: 10.1002/jor.22393
PMCID: PMC5113956
PMID: 23843150
Abstract
Medial meniscus destabilization (MMD) is a surgical insult technique for modeling osteoarthritis (OA) by replicating chronic abnormal cartilage loading in animal joints
in vivo
. The present study aimed to characterize the immediate biomechanical effects (
ex vivo
) and short-term histological consequences (
in vivo
) of MMD in the rabbit knee. In a compressive loading test, contact stress distribution in the medial compartment was measured in eight cadaver rabbit knees, initially with all major joint structures uninjured (Baseline), after MMD, and finally after total medial meniscectomy (TMM). Similarly, the effects on sagittal joint stability were determined in an anterior-posterior drawer test. These biomechanical (
ex vivo)
data indicated that both MMD and TMM caused significant (p < 0.001), distinct (> 1.5-fold) elevation of peak local contact stress in the medial compartment, while leaving whole-joint stability nearly unchanged. Histological consequences
in vivo
were assessed in a short-term (8-week) survival series of MMD or TMM (5 animals for each group), and both caused moderate cartilage degeneration in the medial compartment. The MMD insult, which is feasible through posterior arthrotomy alone, is as effective as TMM for modeling injurious-level chronic abnormal cartilage loading in the rabbit knee medial compartment
in vivo
, while minimizing potential confounding effects from whole-joint instability.
Details
- Title: Subtitle
- Replication of Chronic Abnormal Cartilage Loading by Medial Meniscus Destabilization for Modeling Osteoarthritis in the Rabbit Knee In Vivo
- Creators
- Marut Arunakul - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, Iowa, USAYuki Tochigi - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, Iowa, USAJessica E Goetz - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, Iowa, USABryce W Diestelmeier - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, Iowa, USAAnneliese D Heiner - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, Iowa, USAM. James Rudert - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, Iowa, USADouglas C Fredericks - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, Iowa, USAThomas D Brown - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, Iowa, USATodd O McKinley - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, Iowa, USA
- Resource Type
- Journal article
- Publication Details
- Journal of orthopaedic research, Vol.31(10), pp.1555-1560
- DOI
- 10.1002/jor.22393
- PMID
- 23843150
- PMCID
- PMC5113956
- NLM abbreviation
- J Orthop Res
- ISSN
- 0736-0266
- eISSN
- 1554-527X
- Grant note
- name: NIH-NIAMS grant, award: P50 AR055533; DOI: 10.13039/100000005, name: US Department of Defense, award: W81XWH-10-1-0864, W81XWH-11-1-0583
- Language
- English
- Date published
- 10/2013
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Orthopedics and Rehabilitation; Craniofacial Anomalies Research Center
- Record Identifier
- 9984040398202771
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