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Replication of HIV-1 in primary monocyte cultures
Journal article   Peer reviewed

Replication of HIV-1 in primary monocyte cultures

Barbara J Potts, Wendy Maury and Malcolm A Martin
Virology (New York, N.Y.), Vol.175(2), pp.465-476
1990
DOI: 10.1016/0042-6822(90)90431-P
PMID: 2183468

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Abstract

A subpopulation of human monocytes began to express surface CD4 during its in vitro cultivation in the presence of recombinant M-CSF and became susceptible to infection with an isolate of HIV-1 [AD-87(M)], originally recovered from a seropositive individual by cocultivation with primary monocytes. Approximately 10% of the adherent cells synthesized CD4; a similar fraction was infectable with AD-87(M) and produced high levels of progeny particles. An interesting feature of the HIV-1 infection of primary monocytes was the detection of gpl60/120 inside but not on the surface of virus-producing cells. The implication of these results on HIV infection in vivo will be discussed.

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