Journal article
Replication protein A: Single-stranded DNA's first responder: Dynamic DNA-interactions allow replication protein A to direct single-strand DNA intermediates into different pathways for synthesis or repair
BioEssays, Vol.36(12), pp.1156-1161
12/2014
DOI: 10.1002/bies.201400107
PMCID: PMC4629251
PMID: 25171654
Abstract
Replication protein A (RPA), the major single-stranded DNA-binding protein in eukaryotic cells, is required for processing of single-stranded DNA (ssDNA) intermediates found in replication, repair, and recombination. Recent studies have shown that RPA binding to ssDNA is highly dynamic and that more than high-affinity binding is needed for function. Analysis of DNA binding mutants identified forms of RPA with reduced affinity for ssDNA that are fully active, and other mutants with higher affinity that are inactive. Single molecule studies showed that while RPA binds ssDNA with high affinity, the RPA complex can rapidly diffuse along ssDNA and be displaced by other proteins that act on ssDNA. Finally, dynamic DNA binding allows RPA to prevent error-prone repair of double-stranded breaks and promote error-free repair. Together, these findings suggest a new paradigm where RPA acts as a first responder at sites with ssDNA, thereby actively coordinating DNA repair and DNA synthesis.
Details
- Title: Subtitle
- Replication protein A: Single-stranded DNA's first responder: Dynamic DNA-interactions allow replication protein A to direct single-strand DNA intermediates into different pathways for synthesis or repair
- Creators
- Ran Chen - Department of Biochemistry; Carver College of Medicine; University of Iowa; Iowa City IA USAMarc S Wold - Department of Biochemistry; Carver College of Medicine; University of Iowa; Iowa City IA USA
- Resource Type
- Journal article
- Publication Details
- BioEssays, Vol.36(12), pp.1156-1161
- DOI
- 10.1002/bies.201400107
- PMID
- 25171654
- PMCID
- PMC4629251
- NLM abbreviation
- Bioessays
- ISSN
- 0265-9247
- eISSN
- 1521-1878
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: P30CA086862
- Language
- English
- Date published
- 12/2014
- Academic Unit
- Radiation Oncology; Biochemistry and Molecular Biology
- Record Identifier
- 9984025296702771
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