Journal article
Repression of the Staphylococcus aureus Accessory Gene Regulator in Serum and In Vivo
Journal of bacteriology, Vol.184(4), pp.1095-1101
02/2002
DOI: 10.1128/jb.184.4.1095-1101.2002
PMCID: PMC134826
PMID: 11807070
Abstract
Subgenomic DNA microarrays were employed to evaluate the expression of the accessory gene regulator (
agr
locus) as well as multiple virulence-associated genes in
Staphylococcus aureus
. Gene expression was examined during growth of
S. aureus
in vitro in standard laboratory medium and rabbit serum and in vivo in subcutaneous chambers implanted in either nonimmune rabbits or rabbits immunized with staphylococcal enterotoxin B. Expression of RNAIII, the effector molecule of the
agr
locus, was dramatically repressed in serum and in vivo, despite the increased expression of secreted virulence factors sufficient to cause toxic shock syndrome (TSS) in the animals. Statistical analysis and clustering of virulence genes based on their expression profiles in the various experimental conditions demonstrated no positive correlation between the expression of
agr
and any staphylococcal virulence factors examined. Disruption of the
agr
locus had only a minimal effect on the expression in vivo of the virulence factors examined. An effect of immunization on the expression of
agr
and virulence factors was also observed. These results suggest that
agr
activation is not necessary for development of staphylococcal TSS and that regulatory circuits responding to the in vivo environment override
agr
activity.
Details
- Title: Subtitle
- Repression of the Staphylococcus aureus Accessory Gene Regulator in Serum and In Vivo
- Creators
- Jeremy M Yarwood - Department of Veterinary Pathobiology and Biomedical Genomics Center, University of Minnesota, St. Paul, MinnesotaJohn K McCormick - Department of Veterinary Pathobiology and Biomedical Genomics Center, University of Minnesota, St. Paul, MinnesotaMichael L Paustian - Department of Veterinary Pathobiology and Biomedical Genomics Center, University of Minnesota, St. Paul, MinnesotaVivek Kapur - Department of Veterinary Pathobiology and Biomedical Genomics Center, University of Minnesota, St. Paul, MinnesotaPatrick M Schlievert - Department of Veterinary Pathobiology and Biomedical Genomics Center, University of Minnesota, St. Paul, Minnesota
- Resource Type
- Journal article
- Publication Details
- Journal of bacteriology, Vol.184(4), pp.1095-1101
- Publisher
- American Society for Microbiology
- DOI
- 10.1128/jb.184.4.1095-1101.2002
- PMID
- 11807070
- PMCID
- PMC134826
- ISSN
- 0021-9193
- eISSN
- 1098-5530
- Language
- English
- Date published
- 02/2002
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001125002771
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