Journal article
Requirement for Nuclear Factor- Kappa B Activation by a Distinct Subset of CD40-Mediated Effector Functions in B Lymphocytes
The Journal of immunology (1950), Vol.162(5), pp.2804-2811
03/01/1999
DOI: 10.4049/jimmunol.162.5.2804
PMID: 10072527
Abstract
CD40 stimulation, which is crucial for generating an effective T-dependent humoral response, leads to the activation of transcription factors NF-AT (nuclear factor of activated T cells), AP-1 (activator protein-1), and NF- Kappa B (nuclear factor- Kappa B). However, which CD40-mediated B cell functions actually require activation of specific transcription factors is unknown. We examined the causal relationship between NF- Kappa B activation and CD40 effector functions by evaluating CD40 functions in the presence of an inducible mutant inhibitory Kappa B alpha (I Kappa B alpha ) superrepressor. I Kappa B alpha AA inhibited nuclear translocation of multiple NF- Kappa B dimers without the complicating effect of depriving cells of NF- Kappa B during development. This approach complements studies that use mice genetically deficient in single or multiple NF- Kappa B subunits. Interestingly, only a subset of CD40 effector functions was found to require NF- Kappa B activation. Both CD40-induced Ab secretion and B7-1 up-regulation were completely abrogated by expression of I Kappa B alpha AA. Surprisingly, up-regulation of Fas, CD23, and ICAM-1 was partially independent, and up-regulation of LFA-1 was completely independent, of CD40-induced NF- Kappa B activation. For the first time, it is clear that distinct transcription factors are required for the dynamic regulation of CD40 functions.
Details
- Title: Subtitle
- Requirement for Nuclear Factor- Kappa B Activation by a Distinct Subset of CD40-Mediated Effector Functions in B Lymphocytes
- Creators
- Yina HsingGail A Bishop
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.162(5), pp.2804-2811
- DOI
- 10.4049/jimmunol.162.5.2804
- PMID
- 10072527
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Language
- English
- Date published
- 03/01/1999
- Academic Unit
- Microbiology and Immunology; President
- Record Identifier
- 9984001109402771
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