Requirement for generation of H2O2 for platelet-derived growth factor signal transduction

Maitrayee Sundaresan; Zu-Xi Yu; Victor J Ferrans; Kaikobad Irani; Toren Finkel

doi:10.1126/science.270.5234.296

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Requirement for generation of H2O2 for platelet-derived growth factor signal transduction

Journal article

Peer reviewed

Requirement for generation of H2O2 for platelet-derived growth factor signal transduction

Maitrayee Sundaresan, Zu-Xi Yu, Victor J Ferrans, Kaikobad Irani and Toren Finkel

Science (American Association for the Advancement of Science), Vol.270(5234), pp.296-299

10/13/1995

DOI: 10.1126/science.270.5234.296

PMID: 7569979

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Abstract

Stimulation of rat vascular smooth muscle cells (VSMCs) by platelet-derived growth factor (PDGF) transiently increased the intracellular concentration of hydrogen peroxide (H2O2). This increase could be blunted by increasing the intracellular concentration of the scavenging enzyme catalase or by the chemical antioxidant N-acetylcysteine. The response of VSMCs to PDGF, which includes tyrosine phosphorylation, mitogen-activated protein kinase stimulation, DNA synthesis, and chemotaxis, was inhibited when the growth factor-stimulated rise in H2O2 concentration was blocked. These results suggest that H2O2 may act as a signal-transducing molecule, and they suggest a potential mechanism for the cardioprotective effects of antioxidants.

Phosphorylation

Signal Transduction

Free Radical Scavengers - pharmacology

Muscle, Smooth, Vascular - virology

Muscle, Smooth, Vascular - metabolism

Protein-Tyrosine Kinases - metabolism

Humans

Phosphotyrosine - metabolism

Adenoviridae - genetics

Muscle, Smooth, Vascular - drug effects

Mitogen-Activated Protein Kinases

Cell Line

Cells, Cultured

Rats

Adenoviridae - physiology

Chemotaxis - drug effects

Platelet-Derived Growth Factor - pharmacology

Muscle, Smooth, Vascular - cytology

Hydrogen Peroxide - metabolism

Catalase - metabolism

Animals

Endopeptidase K

Acetylcysteine - pharmacology

Serine Endopeptidases - metabolism

Mitogen-Activated Protein Kinase 1

Calcium-Calmodulin-Dependent Protein Kinases - metabolism

Mitogen-Activated Protein Kinase 3

Details

Title: Subtitle: Requirement for generation of H2O2 for platelet-derived growth factor signal transduction
Creators: Maitrayee Sundaresan - Cardiology Branch, National Heart, Lung, and Blood Institute (NHLBI), National Institutes of Health (NIH), Bethesda, MD 20892-1650, USA
Zu-Xi Yu
Victor J Ferrans
Kaikobad Irani
Toren Finkel
Resource Type: Journal article
Publication Details: Science (American Association for the Advancement of Science), Vol.270(5234), pp.296-299
Publisher: United States
DOI: 10.1126/science.270.5234.296
PMID: 7569979
ISSN: 0036-8075
eISSN: 1095-9203
Language: English
Date published: 10/13/1995
Academic Unit: Cardiovascular Medicine; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
Record Identifier: 9984047791002771

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