Requirement for intracellular calcium modulation in zebrafish dorsal–ventral patterning

Trudi A Westfall; Beth Hjertos; Diane C Slusarski

doi:10.1016/S0012-1606(03)00209-4

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Requirement for intracellular calcium modulation in zebrafish dorsal–ventral patterning

Journal article

Open access

Peer reviewed

Requirement for intracellular calcium modulation in zebrafish dorsal–ventral patterning

Trudi A Westfall, Beth Hjertos and Diane C Slusarski

Developmental biology, Vol.259(2), pp.380-391

2003

DOI: 10.1016/S0012-1606(03)00209-4

PMID: 12871708

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Abstract

The phosphoinositide (PI) cycle is an important signal transduction pathway that, upon activation, generates intracellular second messengers and leads to calcium release. To determine whether PI cycle-mediated intracellular calcium release is required for body plan formation, we systematically dissect PI cycle function in the zebrafish ( Danio rerio). We inhibit PI cycle function at three different steps and deplete internal calcium stores, demonstrating an impact on endogenous calcium release and Wnt/β-catenin signaling. Inhibition of endogenous calcium modulation induces hyperdorsalized phenotypes in a dose-dependent manner. Ectopic dorsal-signaling centers are generated in PI cycle-inhibited embryos as demonstrated by altered β-catenin subcellular localization and ectopic expression of β-catenin target genes. These results provide evidence that modulation of calcium release is critical for early embryonic patterning and acts by influencing the stabilization of β-catenin protein.

Calcium

β-Catenin

Zebrafish

Dorsal–ventral patterning

Phospholipase C (PLC)

Wnt

Phosphatidylinositol cycle

Inositol 1,4,5-trisphosphate receptor (IP 3R)

Details

Title: Subtitle: Requirement for intracellular calcium modulation in zebrafish dorsal–ventral patterning
Creators: Trudi A Westfall - Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, USA
Beth Hjertos - Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, USA
Diane C Slusarski - Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Developmental biology, Vol.259(2), pp.380-391
DOI: 10.1016/S0012-1606(03)00209-4
PMID: 12871708
NLM abbreviation: Dev Biol
ISSN: 0012-1606
eISSN: 1095-564X
Publisher: Elsevier Inc
Language: English
Date published: 2003
Academic Unit: Iowa Neuroscience Institute; Biology
Record Identifier: 9983992089802771

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