Requirement of Watson-Crick Hydrogen Bonding for DNA Synthesis by Yeast DNA Polymerase η

M. Todd Washington; Sandra A Helquist; Eric T Kool; Louise Prakash; Satya Prakash

doi:10.1128/MCB.23.14.5107-5112.2003

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Requirement of Watson-Crick Hydrogen Bonding for DNA Synthesis by Yeast DNA Polymerase η

Journal article

Peer reviewed

Requirement of Watson-Crick Hydrogen Bonding for DNA Synthesis by Yeast DNA Polymerase η

M. Todd Washington, Sandra A Helquist, Eric T Kool, Louise Prakash and Satya Prakash

Molecular and cellular biology, Vol.23(14), pp.5107-5112

07/2003

DOI: 10.1128/MCB.23.14.5107-5112.2003

PMCID: PMC162216

PMID: 12832493

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http://doi.org/10.1128/MCB.23.14.5107-5112.2003View

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Abstract

Classical high-fidelity DNA polymerases discriminate between the correct and incorrect nucleotides by using geometric constraints imposed by the tight fit of the active site with the incipient base pair. Consequently, Watson-Crick (W-C) hydrogen bonding between the bases is not required for the efficiency and accuracy of DNA synthesis by these polymerases. DNA polymerase η (Polη) is a low-fidelity enzyme able to replicate through DNA lesions. Using difluorotoluene, a nonpolar isosteric analog of thymine unable to form W-C hydrogen bonds with adenine, we found that the efficiency and accuracy of nucleotide incorporation by Polη are severely impaired. From these observations, we suggest that W-C hydrogen bonding is required for DNA synthesis by Polη; in this regard, Polη differs strikingly from classical high-fidelity DNA polymerases.

DNA Dynamics and Chromosome Structure

Details

Title: Subtitle: Requirement of Watson-Crick Hydrogen Bonding for DNA Synthesis by Yeast DNA Polymerase η
Creators: M. Todd Washington - Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, Galveston, Texas 77555-1061
Sandra A Helquist - Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, Galveston, Texas 77555-1061
Eric T Kool - Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, Galveston, Texas 77555-1061
Louise Prakash - Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, Galveston, Texas 77555-1061
Satya Prakash - Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, Galveston, Texas 77555-1061
Resource Type: Journal article
Publication Details: Molecular and cellular biology, Vol.23(14), pp.5107-5112
DOI: 10.1128/MCB.23.14.5107-5112.2003
PMID: 12832493
PMCID: PMC162216
NLM abbreviation: Mol Cell Biol
ISSN: 0270-7306
eISSN: 1098-5549
Publisher: American Society for Microbiology
Language: English
Date published: 07/2003
Academic Unit: Radiation Oncology; Biochemistry and Molecular Biology
Record Identifier: 9984024547802771

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