Requirement of a critical period of transcription for induction of a late phase of LTP

P V Nguyen; T Abel; E R Kandel

doi:10.1126/science.8066450

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Requirement of a critical period of transcription for induction of a late phase of LTP

Journal article

Peer reviewed

Requirement of a critical period of transcription for induction of a late phase of LTP

P V Nguyen, T Abel and E R Kandel

Science (American Association for the Advancement of Science), Vol.265(5175), pp.1104-1107

08/19/1994

DOI: 10.1126/science.8066450

PMID: 8066450

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Abstract

Repeated high-frequency trains of stimuli induce long-term potentiation (LTP) in the CA1 region that persists for up to 8 hours in hippocampal slices and for days in intact animals. This long time course has made LTP an attractive model for certain forms of long-term memory in the mammalian brain. A hallmark of long-term memory in the intact animal is a requirement for transcription, and thus whether the late phase of LTP (L-LTP) requires transcription was investigated here. With the use of different inhibitors, it was found in rat hippocampal slices that the induction of L-LTP [produced either by tetanic stimulation or by application of the cyclic adenosine monophosphate (cAMP) analog Sp-cAMPS (Sp-cyclic adenosine 3',5'-monophosphorothioate)] was selectively prevented when transcription was blocked immediately after tetanization or during application of cAMP. As with behavioral memory, this requirement for transcription had a critical time window. Thus, the late phase of LTP in the CA1 region requires transcription during a critical period, perhaps because cAMP-inducible genes must be expressed during this period.

Pyramidal Cells - metabolism

Transcription, Genetic - drug effects

Electric Stimulation

Rats

Male

Dichlororibofuranosylbenzimidazole - pharmacology

Hippocampus - drug effects

Thionucleotides - pharmacology

Rats, Sprague-Dawley

Cyclic AMP - pharmacology

Evoked Potentials - drug effects

Hippocampus - metabolism

Animals

Cyclic AMP - analogs & derivatives

Long-Term Potentiation - drug effects

Synaptic Transmission - drug effects

In Vitro Techniques

Cyclic AMP - metabolism

Dactinomycin - pharmacology

Details

Title: Subtitle: Requirement of a critical period of transcription for induction of a late phase of LTP
Creators: P V Nguyen - Howard Hughes Medical Institute, New York, NY
T Abel
E R Kandel
Resource Type: Journal article
Publication Details: Science (American Association for the Advancement of Science), Vol.265(5175), pp.1104-1107
Publisher: United States
DOI: 10.1126/science.8066450
PMID: 8066450
ISSN: 0036-8075
eISSN: 1095-9203
Grant note: GM32099 / NIGMS NIH HHS
Language: English
Date published: 08/19/1994
Academic Unit: Molecular Physiology and Biophysics; Psychiatry; Psychological and Brain Sciences; Iowa Neuroscience Institute; Neuroscience and Pharmacology; Biochemistry and Molecular Biology
Record Identifier: 9984065837602771

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