Requirements for Endothelin type-A receptors and Endothelin-1 signaling in the facial ectoderm for the patterning of skeletogenic neural crest cells in zebrafish

Sreelaja Nair; Wei Li; Robert Cornell; Thomas F Schilling

doi:10.1242/dev.02704

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Requirements for Endothelin type-A receptors and Endothelin-1 signaling in the facial ectoderm for the patterning of skeletogenic neural crest cells in zebrafish

Journal article

Open access

Peer reviewed

Requirements for Endothelin type-A receptors and Endothelin-1 signaling in the facial ectoderm for the patterning of skeletogenic neural crest cells in zebrafish

Sreelaja Nair, Wei Li, Robert Cornell and Thomas F Schilling

Development (Cambridge), Vol.134(2), pp.335-345

01/2007

DOI: 10.1242/dev.02704

PMID: 17166927

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Abstract

Genetic studies in mice and zebrafish have revealed conserved requirements for Endothelin 1 (Edn1) signaling in craniofacial development. Edn1 acts through its cognate type-A receptor (Ednra) to promote ventral skeletal fates and lower-jaw formation. Here, we describe the isolation and characterization of two zebrafish ednra genes - ednra1 and ednra2 - both of which are expressed in skeletal progenitors in the embryonic neural crest. We show that they play partially redundant roles in lower-jaw formation and development of the jaw joint. Knockdown of Ednra1 leads to fusions between upper- and lower-jaw cartilages, whereas the combined loss of Ednra1 and Ednra2 eliminates the lower jaw, similar to edn1-/- mutants. edn1 is expressed in pharyngeal arch ectoderm, mesoderm and endoderm. Tissue-mosaic studies indicate that, among these tissues, a crucial source of Edn1 is the surface ectoderm. This ectoderm also expresses ednrA1 in an edn1-dependent manner, suggesting that edn1 autoregulates its own expression. Collectively, our results indicate that Edn1 from the pharyngeal ectoderm signals through Ednra proteins to direct early dorsoventral patterning of the skeletogenic neural crest.

Mutation

Signal Transduction

DNA, Complementary - genetics

Molecular Sequence Data

Phylogeny

Zebrafish - embryology

Neural Crest - metabolism

Base Sequence

Body Patterning

Joints - metabolism

Amino Acid Sequence

Skull - metabolism

Endothelin-1 - genetics

Endothelin-1 - metabolism

Zebrafish Proteins - metabolism

Receptor, Endothelin A - genetics

Zebrafish - genetics

Sequence Homology, Amino Acid

Skull - embryology

Animals

Joints - embryology

Models, Biological

Neural Crest - embryology

Zebrafish - metabolism

Receptor, Endothelin A - metabolism

Mice

Zebrafish Proteins - genetics

Details

Title: Subtitle: Requirements for Endothelin type-A receptors and Endothelin-1 signaling in the facial ectoderm for the patterning of skeletogenic neural crest cells in zebrafish
Creators: Sreelaja Nair - Department of Developmental and Cell Biology, University of California, Irvine, 5210 McGaugh Hall, Irvine, CA 92697-2300, USA
Wei Li
Robert Cornell
Thomas F Schilling
Resource Type: Journal article
Publication Details: Development (Cambridge), Vol.134(2), pp.335-345
DOI: 10.1242/dev.02704
PMID: 17166927
NLM abbreviation: Development
ISSN: 0950-1991
eISSN: 1477-9129
Publisher: England
Grant note: R01 NS041353 / NINDS NIH HHS R01 DE013828 / NIDCR NIH HHS DE-13828 / NIDCR NIH HHS NS-41353 / NINDS NIH HHS
Language: English
Date published: 01/2007
Academic Unit: Anatomy and Cell Biology; Dental Research
Record Identifier: 9984025421202771

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