Journal article
Requirements for Ion and Solute Transport, and pH Regulation During Enamel Maturation
Journal of cellular physiology, Vol.227(4), pp.1776-1785
04/2012
DOI: 10.1002/jcp.22911
PMCID: PMC3373187
PMID: 21732355
Abstract
Transcellular bicarbonate transport is suspected to be an important pathway used by ameloblasts to regulate extracellular pH and support crystal growth during enamel maturation. Proteins that play a role in amelogenesis include members of the ABC transporters (SLC gene family and CFTR). A number of carbonic anhydrases (CAs) have also been identified. The defined functions of these genes are likely interlinked during enamel mineralization. The purpose of this study is to quantify relative mRNA levels of individual SLC, Cftr, and CAs in enamel cells obtained from secretory and maturation stages on rat incisors. We also present novel data on the enamel phenotypes for two animal models, amutant porcine(CFTR-ΔF508) and the NBCe1-null mouse.Our data show that two SLCs(AE2 and NBCe1),
Cftr
,and Car2, Car3,Car6,and Car12 are all significantly up-regulated at the onset of the maturation stage of amelogenesis when compared to the secretory stage. The remaining SLCs and CA gene transcripts showed negligible expression or no significant change in expression from secretory to maturation stages. The enamel of
Cftr
-ΔF508 adult pigs was hypomineralized and showed abnormal crystal growth. NBCe1-null mice enamel was structurally defective and had a marked decrease in mineral content relative to wild-type. These data demonstrate the importance of many non-matrix proteins to amelogenesis and that the expression levels of multiple genes regulating extracellular pH are modulated during enamel maturation in response to an increased need for pH buffering during hydroxyapatite crystal growth.
Details
- Title: Subtitle
- Requirements for Ion and Solute Transport, and pH Regulation During Enamel Maturation
- Creators
- RODRIGO S LACRUZ - Center for Craniofacial Molecular Biology, Herman Ostrow School of Dentistry, University of Southern California, Los Angeles, CaliforniaCHARLES E SMITH - Facility for Electron Microscopy Research, Department of Anatomy and Cell Biology and Faculty of Dentistry, McGill University, Montreal, Quebec, CanadaPIERRE MOFFATT - Genetics Unit, Shriners Hospital for Children, Montreal, Quebec, CanadaEUGENE H CHANG - Department of Otolaryngology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IowaTIMOTHY G BROMAGE - Department of Biomaterials and Biomimetics, New York University College of Dentistry, New York, New YorkPABLO BRINGAS - Center for Craniofacial Molecular Biology, Herman Ostrow School of Dentistry, University of Southern California, Los Angeles, CaliforniaANTONIO NANCI - Laboratory for the Study of Calcified Tissues and Biomaterials, Faculté de Médecine Dentaire, Université de Montréal, Montreal, Quebec, CanadaSANJEEV K BANIWAL - Department of Biochemistry and Molecular Biology, Keck School of Medicine, University of Southern California, Los Angeles, CaliforniaJOSEPH ZABNER - Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IowaMICHAEL J WELSH - Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IowaIRA KURTZ - David Geffen School Medicine at the University of California at Los Angeles, Los Angeles, CaliforniaMICHAEL L PAINE - Center for Craniofacial Molecular Biology, Herman Ostrow School of Dentistry, University of Southern California, Los Angeles, California
- Resource Type
- Journal article
- Publication Details
- Journal of cellular physiology, Vol.227(4), pp.1776-1785
- DOI
- 10.1002/jcp.22911
- PMID
- 21732355
- PMCID
- PMC3373187
- NLM abbreviation
- J Cell Physiol
- ISSN
- 0021-9541
- eISSN
- 1097-4652
- Grant note
- R01 DE013404 || DE / National Institute of Dental and Craniofacial Research : NIDCR R01 DE019629 || DE / National Institute of Dental and Craniofacial Research : NIDCR
- Language
- English
- Date published
- 04/2012
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Anatomy and Cell Biology; Neurosurgery; Internal Medicine
- Record Identifier
- 9984020637602771
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