Journal article
Resident synovial macrophages in synovial fluid: Implications for immunoregulation
Clinical immunology (Orlando, Fla.), Vol.271, 110422
02/2025
DOI: 10.1016/j.clim.2024.110422
PMCID: PMC12515479
PMID: 39701169
Abstract
Resident synovial macrophages (RSMs) are anti-inflammatory, self-renewing macrophages that provide physical immune sequestration of the joint space from the peripheral immune system. Increased permeability of this structure is associated with peripheral immune cells in the synovial fluid (SF). Direct measures of synovial barrier integrity are possible with tissue histology, but after barrier breakdown, if these cells perpetuate or initiate chronic inflammation in SF remains unknown. We sought to identify RSM in human SF as an indirect measure of synovial barrier integrity. To validate findings, we created a novel ex vivo explant model using human synovium. scRNA-seq revealed these SF RSMs upregulated pro-fibrotic and pro-osteoclastic pathways in inflammatory arthritis, but not septic arthritis. Increased frequencies of RSMs in SF was associated with increased sRANKL regardless of underlying pathology. These findings suggest the frequency of RSMs in SF may correlate with synovial barrier damage and in turn, potential damage to joint structures.
Details
- Title: Subtitle
- Resident synovial macrophages in synovial fluid: Implications for immunoregulation
- Creators
- Karen I Cyndari - University of IowaBreanna M Scorza - University of IowaZeb R Zacharias - University of IowaDanielle Pessôa-PereiraLeela Strand - Harvard University PressKurayi Mahachi - University of IowaJuan Marcos Oviedo - University of UtahLisa Gibbs - University of UtahKatherine L Butler - Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, IA, United States of AmericaGraham Ausdal - University of IowaDylan Hendricks - University of IowaRika Yahashiri - Williams CollegeJacob M Elkins - University of IowaTrevor Gulbrandsen - University of IowaAndrew R Peterson - University of IowaMichael C Willey - University of IowaKeke C Fairfax - University of UtahChristine A Petersen - The Ohio State University
- Resource Type
- Journal article
- Publication Details
- Clinical immunology (Orlando, Fla.), Vol.271, 110422
- DOI
- 10.1016/j.clim.2024.110422
- PMID
- 39701169
- PMCID
- PMC12515479
- NLM abbreviation
- Clin Immunol
- ISSN
- 1521-7035
- eISSN
- 1521-7035
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE; SAN DIEGO
- Grant note
- Herbert N. Hultgren Award (Wilderness Medicine Society)University of Iowa Physician-Scientist Training ProgramUniversity of Iowa Department of Emergency Medicine
This work was supported by funding from the Herbert N. Hultgren Award (Wilderness Medicine Society), the University of Iowa Physician-Scientist Training Program, and the University of Iowa Department of Emergency Medicine. Study sponsors were not involved with study content or decision to submit.
- Language
- English
- Electronic publication date
- 12/17/2024
- Date published
- 02/2025
- Academic Unit
- Stead Family Department of Pediatrics; Epidemiology; Emergency Medicine; Orthopedics and Rehabilitation; Obstetrics and Gynecology; Injury Prevention Research Center; Holden Comprehensive Cancer Center; General Pediatrics and Adolescent Medicine
- Record Identifier
- 9984758188302771
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